Secondary Antibodies

Human Lambda Light Chain (free and bound) mouse monoclonal antibody, clone NI 412, NI 268, Azide Free

Applications To identify the light chain type of immunoglobulins or free light chains in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, Indirect Immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgE (Epsilon chain specific) mouse monoclonal antibody, clone B3102E8, Purified

Applications ELISA (Recommended as a Detection Antibody).
Western blotting.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone NI 307, Purified

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgE Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation Unconjugated

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Purified

Applications Flow Cytometry: Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA
FLISA
Immunohistochemistry (frozen sections).
Reactivities Mouse
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone NI 179, Purified

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, immunoblotting, immunoprecipitation, indirect immunoperoxidase and immunofluorescence
General Recommended Dilutions:
Histochemical use: 1/50-1/200
Dilutions may be prepared by adding PBS, pH 7.2.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone NI 179, Ascites

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. The optimum working
dilution is an assay-related characteristic and should always be determined by titration.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1,000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Purified

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of
cytoplasmic IgG, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Ascites

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), Ascites

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Azide Free

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG2, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards. 
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), Purified

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgD mouse monoclonal antibody, clone NI 158, Ascites

Applications To identify the presence of IgD in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgD and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/25 upwards.
Western blot: from 1/50 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Purified

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmuno assay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Ascites

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, Purified

Applications Flow Cytometry and Immunofluorescence Microscopy:
Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.
Reactivities Rat
Conjugation Unconjugated