Secondary Antibodies

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Human IgA Mouse Monoclonal Antibody [Clone ID: NI 69 (A89-034) and NI 184 (A89-035)]
AM20249AF-N

CNY 5,902.00

5周

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Ascites

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Human IgM (Mu chain, Fab-Region) Mouse Monoclonal Antibody [Clone ID: UHB]
AM08147PU-N

CNY 3,718.00

4周

Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone UHB, Purified

Applications ELISA:  ≤ 1 µg/ml.
Flow Cytometry: ≤ 1 µg/106 cells. 
Immunofluorescence: ≤ 1 µg/ml.
Human B Cell Stimulation: ≤ 10 µg/ml.
Reactivities Human
Conjugation Unconjugated
Rat IgA (heavy chain) Mouse Monoclonal Antibody [Clone ID: MARA-1]
SM1027P

CNY 5,951.00

4周

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Purified

Applications ELISA: 5 µg/ml. This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent and purified Rat IgA (Cat.-No SA013) as antigen.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Rat
Conjugation Unconjugated
Human IgM (Fc-region) Mouse Monoclonal Antibody [Clone ID: CH2]
SM3063P

CNY 2,750.00

5周

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified

Applications Western blot. Recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.
Immunocytochemistry.
ELISA.
Reactivities Human
Conjugation Unconjugated
Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252PU-N

CNY 7,161.00

5周

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Purified

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/500 upwards. 
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Mouse IgD (Fc specific) Goat Polyclonal Antibody
AP21481AF-N

CNY 7,161.00

5周

Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Reactivities Mouse
Conjugation Unconjugated
Human IgM (Mu chain specific) Mouse Monoclonal Antibody [Clone ID: SA-DA4]
AM08145PU-N

CNY 3,113.00

4周

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Purified

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Immunohistochemistry on Frozen Sections.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated
Human IgG (Fc specific) Mouse Monoclonal Antibody [Clone ID: 4A10]
AM06320SU-N

CNY 5,346.00

5周

Human IgG (Fc specific) mouse monoclonal antibody, clone 4A10, Ascites

Applications ELISA: 1/10000.
Western Blot: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Reactivities Human
Conjugation Unconjugated
Monkey IgA (Secretory component) Goat Polyclonal Antibody
AP31450AF-N

CNY 6,260.00

5周

Monkey IgA (Secretory component) goat polyclonal antibody, Azide Free

Applications Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes.
As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Working dilutions:
Histochemistry: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/200 - 1/1000.
Reactivities Chimpanzee, Monkey
Conjugation Unconjugated
Monkey IgG (Fab specific) Rabbit Polyclonal Antibody
AP31444AF-N

CNY 6,260.00

5周

Monkey IgG (Fab specific) rabbit polyclonal antibody, Azide Free

Applications Can be used for indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Monkey
Conjugation Unconjugated
Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485AF-N

CNY 7,161.00

5周

Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Unconjugated
Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]
AM20255PU-N

CNY 7,161.00

5周

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Purified

Applications To identify the presence of IgG3 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG3, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.  
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Goat IgG (H+L chain) Chicken Polyclonal Antibody
AP31796PU-N

CNY 2,363.00

5周

Goat IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Goat
Conjugation Unconjugated
Canine IgG (H+L chain) Goat Polyclonal Antibody
AP31541AF-N

CNY 3,402.00

5周

Canine IgG (H+L chain) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivities Canine
Conjugation Unconjugated
Human IgG (Fc heavy chain specific) Mouse Monoclonal Antibody [Clone ID: EM-07]
AM26025PU-N

CNY 2,750.00

5周

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, Aff - Purified

Applications Immunocytochemistry. 
Western Blotting: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
Flow cytometry: 1-4 μg/ml.
ELISA: The Peroxidase conjugate of this antibody is suitable for detection of Human IgG Fc fragments by ELISA. The antibody EM-07 does not crossreact with IgM.
Reactivities Human
Conjugation Unconjugated