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Mouse IgG rabbit polyclonal antibody, Serum
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Secondary Antibodies
Human IgG rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Immunodiffusion. For Ouchterlony: Sample Size: 15 µl Distance between wells: 5 mm Test time: 4 days at 37°C Antigens tested: Normal human serum, purified human IgG, normal mouse serum. Results: Titre with NHS > 1:1000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Canine IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/4000. |
| Reactivities | Canine |
| Conjugation | Biotin |
Mouse IgG (H+L chain) porcine polyclonal antibody, Serum
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Canine IgG (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical use for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Biotin |
Canine IgA (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/500. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Biotin |
Rabbit IgA goat polyclonal antibody, Purified
| Applications | ELISA: 1/100 - 1/1000. Immunodiffusion. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Goat IgM rabbit polyclonal antibody, Purified
| Applications | ELISA: 1/100 - 1/1000. Immunodiffusion. |
| Reactivities | Goat |
| Conjugation | Unconjugated |
Human IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | This antiserum is intended to detect and identify IgE in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Antibody titre: Precipitin titre not less than 1/16 when tested against an appropriate reference in agar immunodiffusion block titration. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgD (Fc specific) goat polyclonal antibody, Azide Free
| Applications | This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, Purified
| Applications | ELISA (1/100-1/10000). Immunodiffusion. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Feline IgG (Fc specific) goat polyclonal antibody, Aff - Purified
| Applications | ELISA: 1/100-1/10,00. Immunodiffusion. |
| Reactivities | Feline |
| Conjugation | Unconjugated |
Porcine IgA goat polyclonal antibody, Aff - Purified
| Applications | ELISA: 1/100-1/1000. Immunodiffusion. |
| Reactivities | Porcine |
| Conjugation | Unconjugated |
Bovine IgG2 sheep polyclonal antibody, Aff - Purified
| Applications | ELISA: 1/100 - 1/10000. Immunodiffusion. |
| Reactivities | Bovine |
| Conjugation | Unconjugated |
Equine IgG goat polyclonal antibody, Aff - Purified
| Applications | ELISA: 1/100-1/1000. Immunodiffusion. |
| Reactivities | Equine |
| Conjugation | Unconjugated |
