Secondary Antibodies

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, Purified

Applications ELISA: 1/4,000-1/8,000.
Flow Cytometry.
Reactivities Rabbit
Conjugation Unconjugated

IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Second step reagent for rat IgG2c Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Low Endotoxin

Applications Flow Cytometry (See Ref.1-4).
Functional Assays.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Purified

Applications Flow Cytometry. (See Ref.1-5)
Reactivities Mouse
Conjugation Unconjugated

Human IgD mouse monoclonal antibody, clone IA6-2, Purified

Applications Flow cytometry.
Reactivities Human
Conjugation Unconjugated