Secondary Antibodies

Human IgE mouse monoclonal antibody, clone BE5, Purified

Applications ELISA.
Flow Cytometry: 5-10 µg/ml.
Reactivities Human
Conjugation Unconjugated

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified

Applications Western blot. Recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.
Immunocytochemistry.
ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 904, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Purified

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Unconjugated

Human IgG (gamma chain specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Human
Conjugation Unconjugated

Human IgG (F(ab)2 specific) mouse monoclonal antibody, clone 4A11, Purified

Applications ELISA.
Western blotting.
Flow Cytometry.

Has been described for previous lots to work in Immunofluorescence.
Reactivities Human
Conjugation Unconjugated

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), HRP

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation HRP

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Purified

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/500 upwards. 
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Purified

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Immunohistochemistry on Frozen Sections.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone 4A10, Ascites

Applications ELISA: 1/10000.
Western Blot: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Reactivities Human
Conjugation Unconjugated

Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:500 - 1:2,500
ELISA: 1:250,000
Reactivities Human
Conjugation HRP

Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:500 - 1:2,500
ELISA: 1:250,000
Reactivities Human
Conjugation HRP

Human IgE goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Human, Monkey
Conjugation HRP

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Biotin

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/100 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin