Secondary Antibodies

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Purified

Applications ELISA: 5 µg/ml. This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent and purified Rat IgA (Cat.-No SA013) as antigen.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Rat
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone BE5, Purified

Applications ELISA.
Flow Cytometry: 5-10 µg/ml.
Reactivities Human
Conjugation Unconjugated

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified

Applications Western blot. Recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.
Immunocytochemistry.
ELISA.
Reactivities Human
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgE Antibody, Clone RM122

Applications ELISA: 10ng/well - 100ng/well for Capture;, 0.01ug/mL - 0.1ug/mL for Detection;, ICC: 0.5ug/mL-2ug/mL, IHC: 0.5ug/mL-2ug/mL
Reactivities Human IgE
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 904, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Purified

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Unconjugated

Rabbit Monoclonal Anti-Mouse IgG1 antibody, clone RM106

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB: 0.1ug/mL-0.5ug/mL
Reactivities Mouse IgG1
Conjugation Unconjugated

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, Purified

Applications ELISA: 5µg/ml as coating antibody.
Reactivities Mouse
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgM antibody, clone RM121

Applications ELISA: 50ng/well – 200ng/well (for Capture);0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgM
Conjugation Unconjugated

IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425

Applications Tested for immunohistochemistry (IHC), ELISA and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:2000) IHC, paraffin sections: 0.25µg/ml (1:800); Proteinase K pretreatment for antigen retrieval is recommended. Western Blot: 0.05 – 0.5µg/ml. ELISA: 20 - 50ng/ml (EC50 of 1ng/ml illustrated below) Optimal dilutions should be determined by the end user.
Reactivities Porcine
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgA antibody, clone RM128

Applications ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgA
Conjugation Unconjugated

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, Purified

Applications ELISA: 1/4,000-1/8,000.
Flow Cytometry.
Reactivities Rabbit
Conjugation Unconjugated

Human IgG (F(ab)2 specific) mouse monoclonal antibody, clone 4A11, Purified

Applications ELISA.
Western blotting.
Flow Cytometry.

Has been described for previous lots to work in Immunofluorescence.
Reactivities Human
Conjugation Unconjugated

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Purified

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/500 upwards. 
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Purified

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Immunohistochemistry on Frozen Sections.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated