Secondary Antibodies

Rat IgG Fc specific rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation HRP

Rat IgG (H+L chain), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:7,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation AP

Rat IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/500,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
Reactivities Rat
Conjugation Biotin

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/1000 - 1/2000.
Immunohistochemistry on frozen sections: 1/50.
Immunohistochemistry on paraffin sections: 1/50.
Immunohistochemistry on resin sections: 1/50.
Reactivities Rat
Conjugation HRP

mIgG (1,2a,2b,3) Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9F11

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse, Rat
Conjugation Unconjugated

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