Secondary Antibodies

Bovine IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation AP

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:11,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation AP

Bovine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot 1:1,000 - 1:10,000), ELISA (1:10,000 - 1:50,000=, immunoperoxidase electron microscopy and immunohistochemistry (1:500 - 1;2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1:1,000-10,000), ELISA (1:10,000 - 1:50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP

Bovine IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product.
Reactivities Bovine
Conjugation AP

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Immunoblotting.
ELISA.
Immunohistochemistry.
Immunofluorescence.
Reactivities Bovine
Conjugation Biotin

Bovine IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/15,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Reactivities Bovine
Conjugation Biotin

Human IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1:10,000-1:50,000.
Western Blot: 1:1,000-1:5,000.
Immunohistochemistry: 1:500-1:3,000.
Reactivities Human
Conjugation Unconjugated

Porcine IgM (heavy chain) rabbit polyclonal antibody, Purified

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10000-1/50000.
Immunofluorescence: 1/1000-1/5000.
Flow Cytometry: 1/500-1/2500.
Reactivities Porcine
Conjugation Unconjugated

Human IgG rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/200-1/500 dilution in an ABC method (30 minutes at room temperature).
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgG rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/200-1/500 dilution in an ABC method (30 minutes at room temperature).
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, HRP

Applications This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, Western-blot, Immunoprecipitation and more generally immunoassays.
Anti-Mouse IgG2a heavy chain antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/90,000
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Human IgD (F(ab)2 Fragment) rabbit polyclonal antibody, Purified

Applications ELISA
Reactivities Human
Conjugation Unconjugated

Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in swine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electrondense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Porcine
Conjugation HRP