Secondary Antibodies

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, HRP

Applications This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, Western-blot, Immunoprecipitation and more generally immunoassays.
Anti-Mouse IgG2a heavy chain antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/90,000
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in swine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electrondense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Porcine
Conjugation HRP

Sheep IgM (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000.
Reactivities Sheep
Conjugation HRP

Sheep IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in sheep serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000.
Reactivities Sheep
Conjugation HRP

Sheep IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Reactivities Sheep
Conjugation HRP

Sheep IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8.000.
Reactivities Sheep
Conjugation HRP

Canine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Reactivities Dog
Conjugation HRP

Feline IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Reactivities Feline
Conjugation HRP

Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/150,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Pig
Conjugation HRP

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, HRP

Applications Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western blotting as well as other anti IgG1 antibody based assays.
Recommended Dilutions:
ELISA: 1/300,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Human IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000
Western Blot: 1/5,000-1/25,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Human
Conjugation HRP

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Goat
Conjugation HRP

Mouse IgG F(ab')2 Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

Sheep IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation HRP

Golden Syrian Hamster IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Conjugation HRP