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Secondary Antibodies
Goat IgG (H+L chain) rabbit polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/2,000-1/13,000. Western blot: 1/500-1/2,500. Immunohistochemistry: 1/200-1/1,000. |
| Reactivities | Goat |
| Conjugation | AP |
Monkey IgG (Fab specific) rabbit polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical staining to identify and measure free or Ig bound subunits of both kappa and lambda type at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Monkey |
| Conjugation | Biotin |
Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/75,000-1/500,000. Western Blot: 1/10,000-1/50,000. Immunohistochemistry: 1/1,000-1/10,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
