Secondary Antibodies

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Sheep IgM (H+L chain) Rabbit Polyclonal Antibody
AP33084FC-N

CNY 5,486.00

5周

Sheep IgM (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Frozen Sections.
Can be used to identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC
Sheep IgG (H+L chain) Rabbit Polyclonal Antibody
AP33082AF-N

CNY 2,825.00

5周

Sheep IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.
Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated
Sheep IgG (Fc specific) Rabbit Polyclonal Antibody
AP33081FC-N

CNY 7,161.00

5周

Sheep IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
(In)direct Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Can be used In direct staining of cytoplasmic IgG in fixed sheep cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC
Duck IgG (H+L chain) Rabbit Polyclonal Antibody
AP33057FC-N

CNY 6,341.00

5周

Duck IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
(In)direct Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of duck origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:  1/20-1/80.
Reactivities Duck
Conjugation FITC
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
AP31662PU-N

CNY 6,083.00

5周

Mouse IgG (H+L chain) rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.
Ouchterlony Test:
Sample size: 15 µl.
Distance between wells: 5 mm.
Test Time: 5 days.
Temperature: 37°C.
Samples Tested: Normal mouse Serum (NMS), Purified mouse IgG, Normal human serum (NHS), Purified human IgG.
Results:
Specificity: a single band against NMS was detected at dilutions of 1/4 and 1/8 which is homogeneous with band to purified mouse IgG.
No bands were detected against human IgG at 5 mg/ml or NHS at 1/4 dilution.
Protein content: Biuret Assay 14 mg/ml.
Reactivities Mouse
Conjugation Unconjugated
Human IgG Rabbit Polyclonal Antibody
AP31649PU-N

CNY 6,083.00

5周

Human IgG rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.
Reactivities Human
Conjugation Unconjugated
Human IgD (F(ab)2 Fragment) Rabbit Polyclonal Antibody
AP05392PU-N

CNY 3,500.00

4周

Human IgD (F(ab)2 Fragment) rabbit polyclonal antibody, Purified

Applications ELISA
Reactivities Human
Conjugation Unconjugated
Rat IgG2c Rabbit Polyclonal Antibody
AP05381PU-N

CNY 3,200.00

4周

Rat IgG2c rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Human, Rat
Conjugation Unconjugated
Rat IgG2b Rabbit Polyclonal Antibody
AP05380PU-N

CNY 3,200.00

4周

Rat IgG2b rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated
Goat IgG (Fc specific) Rabbit Polyclonal Antibody
R1319P

CNY 2,583.00

4周

Goat IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications ELISA:1/20,000-1/100,000.
Immunohistochemistry:1/1,000-5,000.
WB:1/2,000-10,000.
Reactivities Goat
Conjugation Unconjugated
Goat IgG (H+L chain) Rabbit Polyclonal Antibody
R1317P

CNY 2,520.00

4周

Goat IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/800,000. 
Western blot: 1/10,000-1/60,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Goat
Conjugation Unconjugated
Goat IgG (H+L chain) Rabbit Polyclonal Antibody
R1317B

CNY 2,657.00

4周

Goat IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other assays using streptavidin/avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/1,00,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Goat
Conjugation Biotin
Canine IgG (Fc specific) Rabbit Polyclonal Antibody
R1311HRP

CNY 3,759.00

4周

Canine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Reactivities Dog
Conjugation HRP
Feline IgG (H+L chain) Rabbit Polyclonal Antibody
R1307HRP

CNY 2,919.00

4周

Feline IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Reactivities Feline
Conjugation HRP
Bovine IgG (Fc specific) Rabbit Polyclonal Antibody
R1305P

CNY 2,919.00

4周

Bovine IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications ELISA: 1:20,000 - 1:100,000.
Immunohistochemistry: 1:1,000 - 1,5,000.
Western blotting: 1:2,000 - 1;10,000. 
Suitable for most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Reactivities Bovine
Conjugation Unconjugated