Secondary Antibodies

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Sheep IgG (Fc specific) Rabbit Polyclonal Antibody
AP33081BT-N

CNY 7,161.00

5周

Sheep IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
Can be used In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in sheep serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000.
Reactivities Sheep
Conjugation Biotin
Duck IgG (H+L chain) Rabbit Polyclonal Antibody
AP33057AF-N

CNY 3,402.00

5周

Duck IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.
Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Antibody titre: Precipitin titre not less than 1/128 when tested against normal Duck serum in agar block titration.
Reactivities Duck
Conjugation Unconjugated
Duck IgG (H+L chain) Rabbit Polyclonal Antibody
AP33057BT-N

CNY 3,996.00

5周

Duck IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications ELISA.
Dot blot. 
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of duck origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 
Histochemical and Cytochemical Use: 1/10-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000.
Reactivities Duck
Conjugation Biotin
Monkey IgG (H+L chain) Rabbit Polyclonal Antibody
AP31448FC-N

CNY 6,341.00

5周

Monkey IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC
Bovine IgG (H+L chain) Rabbit Polyclonal Antibody
R1304P

CNY 2,657.00

4周

Bovine IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Bovine
Conjugation Unconjugated
Chicken IgG / Chicken IgY (H+L chain) Rabbit Polyclonal Antibody
R1300F

CNY 2,741.00

4周

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Assay Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-5,000.
Reactivities Chicken
Conjugation FITC
Human IgG Rabbit Polyclonal Antibody
DP023-05

CNY 3,350.00

4周

Human IgG rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/200-1/500 dilution in an ABC method (30 minutes at room temperature).
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated
Human IgG Rabbit Polyclonal Antibody
DP023

CNY 5,544.00

4周

Human IgG rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/200-1/500 dilution in an ABC method (30 minutes at room temperature).
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated
F(ab')2 Goat IgG (H&L) Antibody Texas Red™ Conjugated
TA398133

CNY 3,188.00

4周

F(ab')2 Goat IgG (H&L) Antibody Texas Red™ Conjugated

Applications IF: 1:500-1:2,500
Reactivities Goat
Conjugation Texas Red
Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Texas Red™ Conjugated
TA397910

CNY 3,713.00

4周

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Texas Red™ Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:1,000 - 1:5,000
FLISA: 1:20,000 - 1:100,000
Conjugation Texas Red
Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Rhodamine Conjugated
TA397904

CNY 2,756.00

4周

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Rhodamine Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:1,000 - 1:5,000
FLISA: 1:20,000 - 1:100,000
Conjugation TRITC
F(ab')2 Sheep IgG (H&L) Antibody Rhodamine Conjugated
TA398240

CNY 3,188.00

4周

F(ab')2 Sheep IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:500-1:2,500
Conjugation TRITC
F(ab')2 Chicken IgG (H&L) Antibody Texas Red™ Conjugated
TA398118

CNY 3,188.00

4周

F(ab')2 Chicken IgG (H&L) Antibody Texas Red™ Conjugated

Applications IF: 1:500-1:2,500
Conjugation Texas Red
F(ab')2 Bovine IgG (H&L) Antibody Texas Red™ Conjugated
TA398105

CNY 3,188.00

4周

F(ab')2 Bovine IgG (H&L) Antibody Texas Red™ Conjugated

Applications IF: 1:500-1:2,500
Conjugation Texas Red
Bovine IgG (Fc specific) Rabbit Polyclonal Antibody
R1305AP

CNY 4,031.00

4周

Bovine IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation AP