Secondary Antibodies

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 is suggested for this product.
Reactivities Human
Conjugation AP

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/30,000 to 1/120,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation Texas Red

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8,500 to 1:72,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation HRP

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin (cat.-no. RA021HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:12,000 to 1:55,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Human IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:16,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation AP

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/1000 - 1/2000.
Immunohistochemistry on frozen sections: 1/50.
Immunohistochemistry on paraffin sections: 1/50.
Immunohistochemistry on resin sections: 1/50.
Reactivities Rat
Conjugation HRP

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Chicken
Conjugation Texas Red

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product, in Western Blot 1:1,000, Immunohistochemistry 1:500.
Reactivities Chicken
Conjugation AP

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivities Chicken
Conjugation Biotin

Donkey IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product.
Reactivities Donkey
Conjugation AP

Guinea pig IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilution(s):
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Guinea Pig
Conjugation AP

Goat IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry or FACS analysis (1/2,000-1/10,000) as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Goat
Conjugation TRITC

Guinea pig IgG (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Guinea Pig
Conjugation TRITC

Guinea pig IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Guinea Pig
Conjugation FITC