Secondary Antibodies

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Cy5

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation Cy5

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, Cy5

Applications Flow Cytometry: < / = 0.1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation Cy5

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation PE

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Cy5

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation Cy5

Feline IgG mouse monoclonal antibody, clone GPB2-2B1, Purified

Applications ELISA: This antibody can bind to virus-bound immune IgG and can therefore detect anti-FeLV, FIV, FCV, FHV, FCoV/FIPV and FPV IgG activity in ELISA.
Western Blot: This antibody detects a band of approximately 50.0kDa on purified Feline IgG under reducing conditions.
Reactivities Feline
Conjugation Unconjugated

Human IgG (Fc CH2 Domain specific) mouse monoclonal antibody, clone 8A4, Purified

Applications ELISA.
Flow cytometry (1:50/1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Human
Conjugation Unconjugated

Monkey IgA mouse monoclonal antibody, clone IgA5-3B, Purified

Applications ELISA.
Western Blot: Detects a band of approximately 55kDa under reducing conditions.
Reactivities Feline, Monkey
Conjugation Unconjugated

Human IgA2 mouse monoclonal antibody, clone A9604D2, Biotin

Applications ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation Biotin

Human IgA2 mouse monoclonal antibody, clone A9604D2, HRP

Applications ELISA: 1/500-1/2000.
Reactivities Human
Conjugation HRP

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, Biotin

Applications ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation Biotin

Rabbit (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.)
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment
Positive control: Polypus nose
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone B537M, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 090-11348, Purified

Applications ELISA.
Recommended antibody pair for Sandwich Immunoassay:
Capture / Detection
AM00919PU-N / AM00918PU-N
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone B1545M, Purified

Applications
This antibody can pair with itself in ELISA, EIA.
Can be used for specific IgM antibodies detection and for total IgM level detection.
Can also be used as a capture antibody for total IgM level detection.
Reactivities Human
Conjugation Unconjugated