Secondary Antibodies

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Human IgM (heavy chain) Mouse Monoclonal Antibody [Clone ID: SPM557]
AM50119PU-S

CNY 5,606.00

5周

Human IgM (heavy chain) mouse monoclonal antibody, clone SPM557, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated
Human IgG (heavy chain) Mouse Monoclonal Antibody [Clone ID: SPM556]
AM50118PU-S

CNY 5,606.00

5周

Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated
Human IgM (heavy chain) Mouse Monoclonal Antibody [Clone ID: IM373]
AM33324PU-S

CNY 5,606.00

5周

Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated
Human IgM (heavy chain) Mouse Monoclonal Antibody [Clone ID: IM260]
AM33323PU-S

CNY 5,606.00

5周

Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated
Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]
AM20259HR-N

CNY 8,794.00

5周

Human IgM mouse monoclonal antibody, clone NI 179, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP
Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]
AM20258HR-N

CNY 8,794.00

5周

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, HRP

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/500
ELISA: from 1/1000 upwards
Western blot: from 1/2000 upwards.
Reactivities Human
Conjugation HRP
Human IgA (Secretory component) Mouse Monoclonal Antibody [Clone ID: NI 194-4 (A89-039)]
AM20261FC-N

CNY 8,794.00

5周

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), FITC

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques Immunofluorescence staining techinqiues.
General Recommended Dilutions:
Histochemical Use: from 1/10 upwards.
Reactivities Human
Conjugation FITC
Human IgG4 (pFC specific) Mouse Monoclonal Antibody [Clone ID: NI 315 (HP 6206)]
AM20257HR-N

CNY 8,794.00

5周

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), HRP

Applications To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.  
ELISA: from 1/500 upwards. 
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation HRP
Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252TC-N

CNY 8,794.00

5周

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), TRITC

Applications To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation TRITC
Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 25-1 (HP 6207)]
AM20254PU-N

CNY 7,161.00

5周

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Purified

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG2, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/500 upwards. 
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Human IgD Mouse Monoclonal Antibody [Clone ID: NI 158]
AM20250PU-N

CNY 7,161.00

5周

Human IgD mouse monoclonal antibody, clone NI 158, Purified

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgD Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100
ELISA: from 1/25 upwards.
Western blot: from 1/50 upwards.
Reactivities Human
Conjugation Unconjugated
Human IgE Mouse Monoclonal Antibody [Clone ID: NI 307]
AM20251AF-N

CNY 5,902.00

5周

Human IgE mouse monoclonal antibody, clone NI 307, Ascites

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgE and immunoblotting.
General Recommended Dilutions:
Histochemical 1/10-1/100.
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation Unconjugated
Human IgA2 Mouse Monoclonal Antibody [Clone ID: NI 512 (A89-038)]
AM20247PU-N

CNY 7,161.00

5周

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Azide Free

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA2, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/200 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Unconjugated
Human IgA2 Mouse Monoclonal Antibody [Clone ID: NI 512 (A89-038)]
AM20247BT-N

CNY 6,175.00

5周

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Biotin

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA2, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/250 upwards. 
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin
Human IgA2 (A2m2 Allotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 194-3 (A89-040)]
AM20248TC-N

CNY 6,175.00

5周

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), TRITC

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA2(m)2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation TRITC