Secondary Antibodies

Human IgD (heavy chain) goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Mouse IgE goat polyclonal antibody, AP

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse
Conjugation AP

Mouse IgM goat polyclonal antibody, APC

Applications Immunofluorescence.
Reactivities Mouse
Conjugation APC

Rabbit IgG Secondary Antibody Agarose

Conjugation Unconjugated

Rat IgG Antibody Agarose

Conjugation Unconjugated

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Guinea Pig
Conjugation FITC

Mouse IgE goat polyclonal antibody, HRP

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse
Conjugation HRP

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/150,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/10,000.
Reactivities Rat
Conjugation Biotin

Golden Syrian Hamster IgG Antibody Agarose

Conjugation Unconjugated

Mouse IgA (H+L chain), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/5,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Guinea Pig IgG Antibody Agarose

Conjugation Unconjugated

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Recommended Dilutions
Immunofluorescence: 1/100-1/250.
Flow Cytometry: 1/100-1/250.
Reactivities Mouse
Conjugation PE

Human IgG F(c) Antibody Agarose

Conjugation Unconjugated

Chicken IgG Antibody Agarose

Conjugation Unconjugated

Human IgG Antibody Agarose

Conjugation Unconjugated