Secondary Antibodies

Bovine IgG F(ab')2 Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:1:50,000
Conjugation FITC

Human IgG F(c) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Human IgG F(ab')2 Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Mouse IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry (1/100-1/250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. 
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Mouse IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Golden Syrian Hamster IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Applications ELISA: 1/20000. 
Western Blot: 1/500-1/2000.
Immunohistochemistry: 1/250-1/1000.
Reactivities Mouse
Conjugation HRP

Human IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Mouse IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, Biotin

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/150,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions
ELISA: 1/20,000-1/100,000.
Immunohistochemistry: 1/1,000-1/5,000.
Western blot: 1/2,000-1/10,000.
Reactivities Mouse
Conjugation Biotin

Rat IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Rabbit IgG F(c) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Mouse IgM (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Guinea Pig IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Porcine IgA goat polyclonal antibody, Biotin

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/10000-1/100000
Reactivities Porcine
Conjugation Biotin