Secondary Antibodies

Rat IgM goat polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Western Blot: 1:1000 - 1:10,000.
Reactivities Rat
Conjugation HRP

Rat IgA goat polyclonal antibody, HRP

Applications ELISA: 1:10,000.
Western Blot: 1:1,000 - 10,000.
Reactivities Rat
Conjugation HRP

Human IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgE in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Antibody titre: Precipitin titre not less than 1/16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Conjugation Unconjugated

Human IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins.
Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Conjugation Unconjugated

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:800,000. 
Western Blot: 1:5,000 - 1:50,000.
Immunohistochemistry: 1:1,000 - 1:5,000.
Reactivities Hamster
Conjugation Unconjugated

Mouse lambda light chain goat polyclonal antibody, FITC

Applications Flow cytometry.
Immunofluorescence.
Reactivities Mouse
Conjugation FITC

Guinea pig IgG (H+L chain) goat polyclonal antibody, HRP, Purified

Applications ELISA (1/1000-1/10,000).
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Guinea Pig
Conjugation HRP

Porcine IgG (H+L chain) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1:200 - 1:2000.
Reactivities Porcine
Conjugation FITC

Guinea pig IgG (H+L chain) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1:200.
Reactivities Guinea Pig
Conjugation FITC

Chicken IgM goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1/200 - 1/2,000.
Reactivities Chicken
Conjugation FITC

Porcine IgG F(c) specific goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on Frozen Sections: 1/20-1/100.
Reactivities Porcine
Conjugation FITC

Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, Purified

Applications ELISA (1/100-1/10000).
Immunodiffusion.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, FITC

Applications Flow cytometry.
Reactivities Chicken
Conjugation FITC

Chicken IgA goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1/200 - 1/2,000.
Reactivities Chicken
Conjugation FITC

Feline IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/10,00.
Immunodiffusion.
Reactivities Feline
Conjugation Unconjugated