Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified
| Applications | Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. For use in ELISA a dilution of 1:3,000-1:12,000 is recommended. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG2b Antibody Pre-adsorbed
| Applications | WB: 1:4,000 - 1:20,000 IHC: 1:2,000 - 1:10,000 ELISA: 1:40,000 - 1:200,000 |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG3 Antibody Pre-adsorbed
| Applications | WB: 1:4,000 - 1:20,000 IHC: 1:2,000 - 1:10,000 ELISA: 1:40,000 - 1:200,000 |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG (H&L) Antibody Pre-Adsorbed
| Applications | WB: 1:10,000 - 1:50,000 IHC: 1:5,000 - 1:25,000 ELISA: 1:100,000 - 1:500,000 |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG (H&L) Antibody Pre-adsorbed
| Applications | WB: 1:2,000 - 1:20,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:50,000 - 1:100,000 |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgM (mu chain) Antibody
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,400 - 1:40,400 |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled secondary antibody for indirect detection of IgE in mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG3 (subclass specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000. Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG fraction is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/150,000. Western Blot: 1/3,000-1/15,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgA (alpha chain specific) goat polyclonal antibody, Aff - Purified
| Applications | Immunoblotting. Flow Cytometry. Immunohistochemistry. Enzyme-Linked-Immunosorbent-Assay (ELISA). Fluorescent-Linked-Immunosorbent-Assay (FLISA). |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Aff - Purified
| Applications | Indirect Immunostaining (In conjunction with mouse monoclonal antibodies). |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG (Fc specific) goat polyclonal antibody, Ig Fraction
| Applications | ELISA: 1/20000-1/100000. Western Blot: 1/2000-1/10000. Immunohistochemistry: 1/1000-1/5,000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
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