Secondary Antibodies

Canine IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2000.
Reactivities Canine
Conjugation Unconjugated

Canine IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/3000.
Reactivities Canine
Conjugation Unconjugated

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, Aff - Purified

Applications Anti-Mouse IgG1 secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
Recommended Dilutions:
ELISA: 1/40,000-1/200,000.
Western Blot: 1/4,000-1/20,000. 
Immunohistochemistry: 1/2,000-1/10,000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM goat polyclonal antibody

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA: 1/2000 - 1/4000.
Western Blot.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2a goat polyclonal antibody

Applications ELISA: 1/2000 - 1/4000.
Reactivities Mouse
Conjugation Unconjugated

Rat IgG1 goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated

Mouse IgG (adsorbed) goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Mouse
Conjugation Unconjugated

Rat IgM goat polyclonal antibody, Purified

Applications ELISA 1ug/ml - 10ug/ml, Western Blotting, Flow Cytometry
Reactivities Rat
Conjugation Unconjugated

Rat IgA goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Immunofluorescence.
Reactivities Reptiles, Rat
Conjugation Unconjugated

Chicken IgM goat polyclonal antibody, Purified

Applications ELISA: (1/100-1/1000).
Immunodiffusion.
Reactivities Chicken
Conjugation Unconjugated

Porcine IgG F(c) specific goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Western Blot: 1/1000-1/30,000.
Immunodiffusion.
Reactivities Porcine
Conjugation Unconjugated

Equine IgM goat polyclonal antibody, Aff - Purified

Applications ELISA.
Immunodiffusion.
Reactivities Equine
Conjugation Unconjugated

Feline IgM goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Feline
Conjugation Unconjugated

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Purified

Applications ELISA.
Flow Cytometry:
5 µg/ml.
Immunoprecipitation: 1-5 µg/ml.
Reactivities Rat
Conjugation Unconjugated

Mouse IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. 
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Reactivities Mouse
Conjugation Unconjugated