Secondary Antibodies

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested for this product. 
Recommended Dilutions: 
ELISA: 1/2000-1/10000. 
Western Blot: 1/500-1/2500. 
Immunochemistry: 1/200-1/1000.
Reactivities Mouse
Conjugation AP

Turkey IgG (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000.
Reactivities Turkey
Conjugation AP

Canine IgM goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Canine
Conjugation AP

Mouse lambda light chain goat polyclonal antibody, AP

Applications ELISA (1/1,000-1/2,000).
Reactivities Mouse
Conjugation AP

Mouse IgE goat polyclonal antibody, AP

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse
Conjugation AP

Monkey IgG (gamma chain specific) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western Blot: 1/500-1/2,500, Dot blot), ELISA (1/500-1/8,000) and Immunohistochemistry (1/200-1/1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Monkey
Conjugation AP

Human IgG (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation AP

Monkey IgM (Mu chain specific) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (1/500-1/3,000; Western or Dot blot), ELISA (1/2,000-1/10,000) and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Monkey
Conjugation AP

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications Suitable for immunoblotting (western or dot blot, 1:500- 1:2,000), ELISA (1:2,000-1:8,000) and Immunohistochemistry (1:200- 1:1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Chicken
Conjugation AP

Rabbit IgG F(ab')2 specific goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation AP

Rabbit IgG (Fc specific) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:3,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation AP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000- 1/8,000.
Western Blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Rabbit
Conjugation AP

Mouse IgA (H+L chain) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Mouse
Conjugation AP

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Reactivities Chicken
Conjugation AP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, AP

Applications Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions:
ELISA: 1:3,000 - 1:13,500.
Western blot 1:500 - 1:2,500.
Reactivities Rabbit
Conjugation AP