Secondary Antibodies

Human IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins.
Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Conjugation Unconjugated

Human IgD (heavy chain) goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/40,000.
IHC: 1/1,000-1/5,000
Western Blot: 1/2,000-1/10,000.
Reactivities Human
Conjugation Unconjugated

Human IgA + IgG + IgM goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA : 1:70,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:1,000 - 1:5,000
Reactivities Human
Conjugation Unconjugated