Secondary Antibodies

Rat IgA goat polyclonal antibody, HRP

Applications ELISA: 1:10,000.
Western Blot: 1:1,000 - 10,000.
Reactivities Rat
Conjugation HRP

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:800,000. 
Western Blot: 1:5,000 - 1:50,000.
Immunohistochemistry: 1:1,000 - 1:5,000.
Reactivities Hamster
Conjugation Unconjugated

Porcine IgG F(c) specific goat polyclonal antibody, Biotin

Applications ELISA: 1/10000-1/100000. 
Western Blot: 1/10000-1/100000.
Reactivities Porcine
Conjugation Biotin

Porcine IgA goat polyclonal antibody, Biotin

Applications ELISA: 1/10000-1/100000. 
Western Blot: 1/10000-1/100000
Reactivities Porcine
Conjugation Biotin

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested for this product. 
Recommended Dilutions: 
ELISA: 1/2000-1/10000. 
Western Blot: 1/500-1/2500. 
Immunochemistry: 1/200-1/1000.
Reactivities Mouse
Conjugation AP

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. 
Recommended Dilutions:
ELISA: 1/10,000. 
Western Blot : 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Mu chain specific), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. 
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Applications ELISA: 1/20000. 
Western Blot: 1/500-1/2000.
Immunohistochemistry: 1/250-1/1000.
Reactivities Mouse
Conjugation HRP