Secondary Antibodies

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/2,000-1/20,000 for Western blot), ELISA (1/180,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Sheep IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Applications WB: 1:1,000 - 1:4,000
IHC: 1:200 - 1:1,000
ELISA: 1:2,000 - 1:8,000
Conjugation Alkaline Phosphatase

Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Reactivities Goat
Conjugation FITC

Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:5,000
IHC: 1:400 - 1:2,000
ELISA: 1:10,000 - 1:20,000
Reactivities Rabbit
Conjugation Alkaline Phosphatase

Goat IgG F(c) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:5,000
IHC: 1500 - 1:2,500
ELISA: 1:5,000 - 1:25,000
Conjugation HRP

Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Applications WB: 1:500 - 1:2,500
IHC: 1:200 - 1:1,000
ELISA: 1:2,000 - 1:10,000
Conjugation Alkaline Phosphatase

Sheep IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Unconjugated

F(ab')2 Sheep IgG (H&L) Antibody Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Unconjugated

F(ab')2 Rabbit IgG (H&L) Antibody Pre-Adsorbed

Applications WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
ELISA: 1:20,000-1:100,000
Reactivities Rabbit
Conjugation Unconjugated

F(ab')2 Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:500 - 1:5,000
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

Rabbit IgG F(c) Antibody

Applications WB: 1:2,000 - 1:20,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:100,000
Conjugation Unconjugated

Rabbit IgG (H&L) Antibody Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:40,000 - 1:160,000
Conjugation Unconjugated

Mouse IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:50,000 - 1:150,000
Conjugation Unconjugated

Rabbit IgG F(c) Antibody Alkaline Phosphatase Conjugated

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:3,000
ELISA: 1:10,000 - 1:20,000
Conjugation Alkaline Phosphatase