Fgf1 (NM_010197) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Phe16-Asp155
Tag	Tag Free
Predicted MW	15.8 kDa
Concentration	lot specific
Purity	>95% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Provided lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl
Storage	Store at -80°C.
Stability	Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Bioactivity	ED50 is less than 0.5 ng/ml as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of Heparin. Specific Activity of 2.0 x 106 IU/mg.
Endotoxin	< 0.1 EU per µg protein as determined by LAL test
Reference Data
RefSeq	NP_034327
Locus ID	14164
UniProt ID	P61148, Q6ZWS1
Refseq Size	3909
Cytogenetics	18 20.74 cM
Refseq ORF	468
Synonyms	Dffrx; Fam; Fgf-1; Fgfa
Summary	Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.[UniProtKB/Swiss-Prot Function]

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