HCV NS4 (1658-1863, 19 kDa) Protein

Specifications

Product Data
Expression Host	E. coli
Concentration	lot specific
Buffer	Presentation State: Purified State: Liquid purified Ig fraction (95% pure by SDS-PAGE and Bradford et al). Buffer System: 8 M urea, 20 mM Tris-HCl, pH 8.0; 10 mM beta-mercaptoethanol. without preservatives.
Preparation	Liquid purified Ig fraction (95% pure by SDS-PAGE and Bradford et al).
Applications	Suitable in Western Blot with a suggested dilution of 1:1,000.
Protein Description	HCV NS4 antigen (recombinant) NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa, denatured. Beta-galactosidase (114 kDa) fused at N-terminus. Reacts strongly with human HCV positive serum.
Storage	Store the antigen at 2-8°C. DO NOT FREEZE!!
Stability	Shelf life: six months from despatch.
Reference Data
Summary	Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.

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