Idnk (1-187, His-tag) Escherichia coli Protein

Specifications

Product Data
Species	Escherichia coli
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
Tag	His-tag
Predicted MW	23.4 kDa
Concentration	lot specific
Purity	>90% by SDS - PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1 mM DTT
Preparation	Liquid purified protein
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Specific: Specific activity is > 80 units/mg, in which one unit will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.
Reference Data
Summary	D-glucote kise, thermosensitive, also known as idnk, D-glucote kise, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokise gntK/gntV family and catalyzes the conversion of ATP and D-glucote to ADP and 6-phospho-D-glucote. This protein is thought to play a role in gender determition, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. Recombint E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventiol chromatography techniques.

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