CD178 / Fas Ligand (His-tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	CHO
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL
Tag	His-tag
Predicted MW	17.9 kDa
Purity	>95% by SDS-PAGE & HPLC analysis
Buffer	Presentation State: Purified State: Lyophilized purified protein from 10 mM Sodium Phosphate, pH 7.5 + 150 mM NaCl Stabilizer: None
Preparation	Lyophilized purified protein
Protein Description	Recombinant human soluble Fas Ligand is a 17.9 kDa protein (175 amino acid residues) comprising the TNF homologous region of FasL plus an eight His-Tag.
Note	Centrifuge vials before opening!
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤ 10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.
Endotoxin	< 0.1 ng per μg of sFas Ligand
Reference Data
RefSeq	NP_000630
Locus ID	356
UniProt ID	P48023, Q53ZZ1
Cytogenetics	1q24.3
Synonyms	ALPS1B; APT1LG1; APTL; CD95-L; CD95L; CD178; FASL; TNFSF6; TNLG1A
Summary	This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Allograft rejection, Apoptosis, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Pathways in cancer, Type I diabetes mellitus

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