AASDH (NM_001286668) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACSF4; LYS2; NRPS998; NRPS1098
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286668, the custom clone sequence may differ by one or more nucleotides ATGAAAAAATGTAATCTAAAGTATATCCTTGTTGAAAAAAAACAAATTAATAAATTTAAATCTTTTCATG AAACATTATTGAACTATGATACATTTACAGTGGAACATAATGACCTAGTGCTCTTCAGACTTCACTGGAA AAATACTGAGGTGAACTTGATGCTAAATGATGGAAAAGAGAAATATGAAAAAGAAAAAATAAAAAGCATA AGTTCTGAGCATGTCAATGAAGAAAAAGCAGAAGAACACATGGATCTGAGGCTAAAGCATTGCTTAGCCT ATGTTCTACATACATCAGGGACTACAGGGATACCGAAGATTGTCAGAGTGCCTCATAAGTGTATAGTACC AAATATCCAGCATTTTCGGGTACTTTTTGACATCACACAAGAAGATGTTTTGTTTCTGGCTTCACCTCTG ACCTTCGATCCTTCTGTTGTGGAAATATTTCTTGCTCTATCAAGTGGTGCCTCTCTGCTTATTGTACCAA CTTCCGTCAAGTTGCTCCCATCAAAATTAGCCAGCGTTCTCTTTTCCCATCATAGAGTGACTGTTTTGCA GGCAACACCAACATTGCTTAGAAGATTTGGATCTCAGCTTATCAAGTCAACTGTTTTGTCAGCCACTACT TCTCTTCGAGTATTAGCCCTTGGTGGTGAAGCGTTTCCATCATTGACAGTTCTCAGAAGCTGGAGAGGAG AAGGCAATAAAACACAAATATTTAATGTTTATGGTATCACAGAGGTATCAAGTTGGGCGACCATTTATAG GATTCCAGAGAAGACTCTTAACTCTACTCTCAAATGTGAATTGCCTGTACAACTGGGATTTCCACTTCTT GGAACAGTAGTTGAAGTCAGAGATACTAATGGCTTCACAATTCAGGAAGGCAGTGGCCAAGTATTTTTAG GTGGCAGAAACAGAGTGTGTTTTCTTGATGATGAAGTGACAGTACCACTTGGCACAATGCGAGCTACAGG AGACTTTGTGACTGTGAAAGATGGAGAGATTTTTTTTTTGGGACGAAAAGACAGTCAGATCAAACGTCAT GGCAAACGTCTTAACATTGAACTTGTGCAACAGGTTGCTGAAGAGCTTCAGCAAGTGGAGTCTTGTGCAG TTACATGGTATAATCAGGAAAAATTAATTCTCTTCATGGTGTCTAAAGATGCTTCAGTAAAAGAATACAT CTTTAAAGAACTGCAGAAATATCTTCCAAGTCATGCAGTCCCGGATGAGCTTGTATTGATCGACTCTCTA CCATTTACATCCCACGGCAAAATTGATGTTTCTGAGTTAAACAAGATATATTTAAACTACATAAACTTGA AGTCTGAGAATAAGCTCAGTGGGAAAGAGGACCTTTGGGAAAAATTACAGTATTTGTGGAAGTCTACTCT GAATCTCCCAGAAGATCTTTTGAGGGTTCCTGATGAGTCACTCTTCTTAAATAGTGGTGGAGATTCCTTA AAGTCCATCCGGCTCCTCAGTGAGATTGAAAAACTTGTTGGTACATCAGTACCTGGGCTTCTGGAAATTA TTCTCAGCAGTTCCATTTTAGAGATTTATAATCACATCCTTCAAACAGTGGTTCCAGATGAAGATGTGAC ATTCAGGAAGAGTTGTGCCACAAAAAGGAAACTCAGCGACATTAATCAAGAGGAAGCCAGTGGAACATCT TTACATCAGAAAGCCATCATGACTTTCACTTGCCACAATGAGATTAATGCTTTTGTTGTACTGAGCAGAG GGAGTCAAATTTTGTCTCTGAATTCCACTAGGTTTTTAACAAAGTTAGGACATTGCTCTTCAGCCTGTCC TTCTGACTCAGTTTCACAGACCAACATTCAAAATTTGAAAGGCTTAAATTCTCCAGTTCTTATTGGGAAG TCAAAAGATCCATCCTGTGTTGCAAAAGTTTCTGAAGAGGGGAAACCTGCGATAGGGACTCAGAAAATGG AGTTACATGTGAGGTGGAGGTCAGACACAGGCAAATGTGTAGATGCTTCACCGCTGGTTGTAATACCCAC TTTTGATAAGTCATCTACAACTGTGTACATTGGTTCCCATTCTCATAGAATGAAGGCAGTTGACTTTTAC TCTGGGAAGGTAAAATGGGAACAGATTTTGGGAGATCGAATTGAATCCTCAGCATGTGTATCTAAGTGTG GAAACTTTATTGTGGTGGGCTGTTATAATGGATTAGTTTATGTTCTGAAAAGTAATAGTGGAGAAAAATA CTGGATGTTTACTACTGAAGATGCTGTCAAAAGCTCGGCAACCATGGATCCAACCACAGGACTCATTTAC ATTGGATCTCATGACCAGCACGCATATGCTTTAGATATTTATAGAAAGAAGTGTGTTTGGAAGTCAAAAT GTGGAGGAACTGTCTTTTCCTCTCCGTGTTTGAACCTGATTCCACATCATTTGTATTTTGCTACATTGGG AGGACTTTTACTGGCTGTAAATCCTGCTACTGGGAACGTTATTTGGAAACATTCCTGTGGAAAACCACTC TTCTCTTCCCCACAATGTTGCTCACAGTATATTTGTATTGGCTGTGTAGATGGGAATTTACTCTGCTTTA CTCACTTTGGAGAACAGGTTTGGCAGTTCTCTACCAGTGGACCAATCTTTTCATCCCCGTGTACCTCACC ATCAGAGCAAAAAATATTTTTTGGTTCCCATGATTGCTTTATCTACTGTTGTAACATGAAAGGTCACCTG CAGTGGAAATTTGAAACTACTTCAAGGGTCTATGCAACACCGTTTGCTTTCCATAACTACAATGGCAGCA ATGAAATGTTGCTGGCAGCAGCATCTACTGATGGGAAAGTGTGGATCTTGGAATCTCAGAGTGGACAATT GCAAAGTGTTTATGAACTTCCTGGAGAAGTCTTCTCTTCTCCTGTGGTCCTGGAATCAATGCTCATTATT GGGTGTAGAGATAATTATGTTTATTGTCTGGATTTATTGGGTGGCAATCAAAAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286668
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286668.1, NP_001273597.1
RefSeq Size	3342 bp
RefSeq ORF	2997 bp
Locus ID	132949
UniProt ID	Q4L235
Protein Pathways	Lysine biosynthesis, Lysine degradation, Metabolic pathways
Gene Summary	This gene encodes a member of the non-ribosome peptide syntesase (NRPS) enzyme family. The encoded protein contains an AMP-binding domain, PP-binding (phosphopantetheine, or pantetheine 4'phosphate-binding) domain and the Pyrrolo-quinoline quinon (PQQ) binding domain. The protein is expressed in several adult tissues. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (2) lacks an alternate exon in the 5' end and uses a downstream start codon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

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