TAF4B (NM_001293725) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SPGF13; TAF2C2; TAFII105
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001293725, the custom clone sequence may differ by one or more nucleotides ATGCCCGCCGGCCTCACCGAACCCGCCGGCGCCGCTCCCCCGGCTGCTGTGAGCGCCTCGGGGACCGTGA CCATGGCCCCGGCCGGGGCGCTGCCGGTGCGGGTGGAGAGCACTCCGGTGGCCCTGGGCGCCGTGACTAA GGCTCCTGTCAGCGTCTGCGTGGAGCCCACGGCGTCCCAGCCCCTGCGGTCCCCCGTGGGGACCCTGGTG ACCAAAGTGGCTCCGGTCAGCGCCCCTCCTAAAGTCAGCAGCGGCCCTAGGCTGCCTGCTCCTCAGATAG TCGCCGTGAAAGCCCCCAACACCACGACAATCCAGTTTCCTGCTAATTTGCAGCTTCCTCCAGGAACCGT TTTGATTAAAAGTAACAGTGGTCCGTTGATGTTGGTATCTCCTCAGCAAACTGTAACAAGAGCCGAGACC ACAAGTAACATAACCTCAAGGCCAGCAGTACCAGCGAATCCTCAAACAGTCAAAATCTGTACAGTGCCGA ACTCTAGCTCACAATTAATCAAGAAAGTGGCAGTGACACCTGTTAAAAAATTGGCACAAATAGGAACTAC TGTGGTAACCACTGTTCCGAAGCCTTCCTCAGTACAATCTGTGGCTGTGCCAACCAGTGTCGTCACAGTT ACTCCTGGAAAGCCATTGAATACTGTAACTACCCTGAAGCCTTCAAGTTTGGGAGCATCATCCACTCCTT CAAATGAGCCCAATCTTAAAGCAGAGAACTCAGCAGCTGTTCAGATTAATCTTTCTCCGACAATGCTAGA AAATGTGAAGAAATGCAAGAACTTCCTTGCAATGTTAATAAAACTAGCATGTAGTGGATCACAGTCCCCA GAAATGGGGCAAAATGTGAAGAAGCTGGTGGAACAACTTTTGGATGCAAAAATCGAAGCAGAAGAATTTA CTAGGAAACTGTATGTTGAACTCAAGTCTTCACCTCAGCCTCACCTGGTTCCTTTTCTTAAGAAAAGCGT GGTTGCCTTACGACAACTTCTGCCTAACTCCCAGAGCTTCATCCAGCAATGTGTTCAGCAGACTTCTAGT GACATGGTCATTGCTACCTGTACTACAACAGTAACAACTTCTCCTGTGGTGACAACTACAGTGTCCTCAA GCCAGTCTGAAAAGTCAATTATTGTTTCTGGAGCAACAGCACCCAGAACTGTGTCAGTGCAAACTTTGAA CCCACTTGCTGGTCCAGTGGGAGCAAAAGCTGGAGTTGTGACACTTCATTCTGTGGGCCCAACTGCTGCA ACAGGAGGAACAACAGCTGGAACTGGTTTGCTTCAGACTTCAAAACCACTTGTGACATCTGTGGCAAACA CAGTGACCACGGTCTCACTGCAACCTGAAAAGCCAGTTGTCTCTGGAACAGCAGTAACACTGTCCCTTCC AGCAGTAACTTTTGGAGAAACTTCAGGTGCAGCTATTTGTCTTCCATCTGTGAAACCTGTTGTTTCTTCT GCTGGGACCACATCTGACAAGCCTGTTATTGGGACTCCAGTTCAAATCAAACTTGCCCAGCCGGGCCCTG TCCTTTCACAACCAGCTGGGATTCCACAGGCAGTTCAAGTCAAGCAACTATTCTCATTGTTTCAGGTAGT TCAGCAGCCTTCAGGAGGCAATGAAAAACAAGTGACCACAATTTCACATTCCTCAACATTGACCATTCAG AAATGTGGACAGAAGACGATGCCAGTGAACACCATAATACCTACTAGTCAGTTTCCTCCAGCTTCCATTC TAAAGCAAATTACTCTGCCTGGAAATAAAATTCTGTCACTTCAAGCATCTCCTACTCAGAAAAATAGAAT AAAAGAGAATGTAACATCATGCTTCCGAGATGAGGATGACATCAATGATGTGACTTCTATGGCAGGGGTC AACCTTAATGAAGAAAATGCCTGCATCTTAGCAACAAACTCTGAATTGGTTGGCACACTCATTCAGTCAT GTAAAGATGAACCATTTCTTTTTATTGGAGCTCTACAAAAGAGAATTTTAGACATTGGTAAAAAGCATGA CATTACAGAACTTAACTCTGATGCTGTGAACTTGATCTCCCAAGCAACACAGGAACGACTACGAGGCCTT CTAGAAAAACTGACTGCAATTGCTCAGCATCGAATGACTACTTACAAGGCAAGTGAAAATTACATCCTGT GTAGTGATACCAGGTCACAGCTCAAATTTCTTGAAAAGCTGGATCAATTGGAGAAGCAGAGAAAGGATTT GGAAGAAAGAGAAATGTTACTTAAGGCAGCCAAGAGTCGTTCTAATAAAGAAGATCCAGAACAGCTGAGA TTAAAGCAGAAAGCCAAAGAGTTACAGCAATTGGAACTTGCACAGATACAGCATAGAGACGCTAATCTCA CAGCTCTTGCAGCTATTGGACCAAGGAAGAAGAGACCACTAGAATCTGGAATTGAGGGCTTAAAAGACAA CCTTCTTGCTTCTGGGACATCCAGCCTGACAGCCACCAAACAGTTGCATCGTCCAAGAATCACGAGAATC TGCCTCAGGGACTTGATATTTTGTATGGAACAGGAACGGGAGATGAAGTATTCTCGAGCTCTATACCTGG CCCTTCTGAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001293725
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293725.1, NP_001280654.1
RefSeq Size	4354 bp
RefSeq ORF	2604 bp
Locus ID	6875
UniProt ID	Q92750
Protein Pathways	Basal transcription factors, Huntington's disease
Gene Summary	TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (1) encodes the longer isoform (1).

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