CRNKL1 (NM_001278628) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CLF; Clf1; CRN; HCRN; MSTP021; SYF3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278628, the custom clone sequence may differ by one or more nucleotides ATGGCGGCCTCCACCGCGGCCGGGAAGCAGCGGATTCCCAAAGTGGCCAAGGTGAAAAACAAAGCCCCGG CTGAGGTACAGATAACTGCTGAACAACTCTTAAGAGAGGCTAAAGAAAGAGAACTTGAGCTTCTTCCACC TCCACCTCAACAGAAGATCACAGATGAAGAAGAATTAAATGATTATAAACTAAGGAAAAGGAAGACTTTT GAAGATAATATAAGAAAAAACAGGACTGTGATTAGTAACTGGATAAAATACGCACAATGGGAAGAAAGCC TAAAGGAGATTCAAAGGGCTCGATCCATATACGAGCGTGCTTTAGATGTAGACTACCGAAATATTACACT CTGGCTGAAATACGCAGAAATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGGGACCGG GCCATAACAACGCTGCCTCGAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAGGAAATGTTGGGAA ACGTTGCCGGTGCCCGGCAGGTGTTTGAGCGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCCTGGCACTC CTACATCAACTTTGAGCTGAGATACAAAGAGGTGGATCGGGCCCGCACCATTTATGAGCGATTTGTCCTC GTGCACCCTGATGTTAAGAACTGGATCAAGTATGCCCGCTTTGAAGAAAAACATGCTTATTTTGCCCATG CACGGAAAGTGTATGAGAGAGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCACCTTTATGTTGC CTTTGCCAAGTTTGAAGAAAATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAGTATGCCCTGGAC AGAATTTCAAAACAAGATGCCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAGAAGTTTGGTGATA GGCGGGGTATTGAAGATATCATTGTGAGCAAACGGAGATTCCAGTACGAAGAAGAAGTGAAGGCGAATCC ACACAATTATGATGCATGGTTTGATTACTTGCGCTTGGTAGAAAGTGACGCAGAAGCTGAAGCCGTGAGA GAAGTCTATGAAAGGGCCATTGCCAATGTCCCACCCATTCAGGAGAAGAGGCACTGGAAGCGCTACATTT ATCTTTGGATCAACTATGCACTCTATGAAGAATTGGAGGCAAAGGATCCTGAGAGGACAAGACAGGTGTA TCAAGCCTCTTTGGAACTAATTCCTCACAAAAAGTTCACATTTGCCAAAATGTGGATACTGTATGCACAG TTTGAAATACGACAGAAGAATCTGTCATTAGCCAGAAGAGCATTGGGAACTTCCATAGGCAAATGTCCAA AGAACAAATTATTTAAAGTTTACATAGAATTGGAGCTACAGCTTCGAGAATTTGACAGATGCCGGAAGCT TTATGAAAAGTTCCTGGAATTTGGACCTGAAAATTGTACCTCATGGATTAAATTCGCTGAATTAGAGACA ATCCTTGGTGATATTGACAGAGCACGGGCAATCTATGAATTAGCCATCAGTCAGCCACGTTTAGACATGC CAGAGGTGCTTTGGAAATCATATATTGATTTTGAAATTGAGCAGGAAGAAACAGAAAGAACACGAAACCT TTACCGGCGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGGATCAGCTTTGCTCAGTTTGAGTTGTCT TCAGGAAAAGAAGGAAGTTTGACTAAATGCAGACAAATTTATGAAGAAGCTAACAAAACCATGCGAAACT GTGAAGAAAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCGAAGTTTTGAAGAAGAATTTGGAACAGC TTCAGATAAGGAGAGAGTAGACAAACTCATGCCAGAGAAAGTCAAGAAGAGAAGAAAGGTCCAGACTGAT GATGGGTCTGATGCAGGCTGGGAAGAATACTTTGATTACATCTTTCCAGAAGATGCTGCCAACCAACCTA ACCTCAAACTCCTGGCCATGGCCAAACTGTGGAAGAAACAGCAGCAGGAAAAGGAGGATGCTGAGCACCA TCCAGATGAGGACGTCGATGAGAGTGAATCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001278628
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278628.1, NP_001265557.1
RefSeq Size	4048 bp
RefSeq ORF	2064 bp
Locus ID	51340
UniProt ID	Q9BZJ0
Protein Pathways	Spliceosome
Gene Summary	The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. This variant represents use of an alternate promoter, compared to variant 1. The encoded isoform (d) is shorter than isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.