Folylpolyglutamate synthase (FPGS) (NM_001288803) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336795 representing NM_001288803. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGCGGGCGCGGAGCCACCTGCGCGCCGCTCTATTCCTGGCAGCGGCGTCTGCGCGCGGCATAACG ACCCAGGTCGCGGCGCGGCGGGGCTTGAGCGCGTGGCCGGTGCCGCAGGAGCCGAGCATGGAGTACCAG GATGCCGTGCGCATGCTCAATACCCTGCAGACCAATGCCGGCTACCTGGAGCAGGTGAAGCGCCAGCGG GGTGACCCTCAGACACAGTTGGAAGCCATGGAACTGTACCTGGCACGGAGTGGGCTGCAGGTGGAGGAC TTGGACCGGCTGAACATCATCCACGTCACTGGGACGAAGGGGAAGGGCTCCACCTGTGCCTTCACGGAA TGTATCCTCCGAAGCTATGGCCTGAAGACGGGATTCTTTAGCTCTCCCCACCTGGTGCAGGTTCGGGAG CGGATCCGCATCAATGGGCAGCCCATCAGTCCTGAGCTCTTCACCAAGTACTTCTGGCGCCTCTACCAC CGGCTGGAGGAGACCAAGGTGGACCTGGCAGTGGTGGAGGTGGGCATTGGCGGGGCTTATGACTGCACC AACATCATCAGGAAGCCTGTGGTGTGCGGAGTCTCCTCTCTTGGCATCGACCACACCAGCCTCCTGGGG GATACGGTGGAGAAGATCGCATGGCAGAAAGGGGGCATCTTTAAGCAAGGTGTCCCTGCCTTCACTGTG CTCCAACCTGAAGGTCCCCTGGCAGTGCTGAGGGACCGAGCCCAGCAGATCTCATGTCCTCTATACCTG TGTCCGATGCTGGAGGCCCTCGAGGAAGGGGGGCCGCCGCTGACCCTGGGCCTGGAGGGGGAGCACCAG CGGTCCAACGCCGCCTTGGCCTTGCAGCTGGCCCACTGCTGGCTGCAGCGGCAGGACCGCCATGGTGCT GGGGAGCCAAAGGCATCCAGGCCAGGGCTCCTGTGGCAGCTGCCCCTGGCACCTGTGTTCCAGCCCACA TCCCACATGCGGCTCGGGCTTCGGAACACGGAGTGGCCGGGCCGGACGCAGGTGCTGCGGCGCGGGCCC CTCACCTGGTACCTGGACGGTGCGCACACCGCCAGCAGCGCGCAGGCCTGCGTGCGCTGGTTCCGCCAG GCGCTGCAGGGCCGCGAGAGGCCGAGCGGTGGCCCCGAGGTTCGAGTCTTGCTCTTCAATGCTACCGGG GACCGGGACCCGGCGGCCCTGCTGAAGCTGCTGCAGCCCTGCCAGTTTGACTATGCCGTCTTCTGCCCT AACCTGACAGAGGTGTCATCCACAGGCAACGCAGACCAACAGAACTTCACAGTGACACTGGACCAGGTC CTGCTCCGCTGCCTGGAACACCAGCAGCACTGGAACCACCTGGACGAAGAGCAGGCCAGCCCGGACCTC TGGAGTGCCCCCAGCCCAGAGCCCGGTGGGTCCGCATCCCTGCTTCTGGCGCCCCACCCACCCCACACC TGCAGTGCCAGCTCCCTCGTCTTCAGCTGCATTTCACATGCCTTGCAATGGATCAGCCAAGGCCGAGAC CCCATCTTCCAGCCACCTAGTCCCCCAAAGGGCCTCCTCACCCACCCTGTGGCTCACAGTGGGGCCAGC ATACTCCGTGAGGCTGCTGCCATCCATGTGCTAGTCACTGGCAGCCTGCACCTGGTGGGTGGTGTCCTG AAGCTGCTGGAGCCCGCACTGTCCCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288803
Insert Size	1686 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288803.1
RefSeq Size	2230 bp
RefSeq ORF	1686 bp
Locus ID	2356
UniProt ID	Q05932
Protein Pathways	Folate biosynthesis, Metabolic pathways
MW	61.6 kDa
Gene Summary	This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This variant has two alternative translational start codons in the same reading frame which encode either a longer, signal-containing mitochondrial protein (isoform c) or a shorter, signal-less cytosolic protein.

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