DNA polymerase mu (POLM) (NM_001284330) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Pol Mu; Tdt-N
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336520 representing NM_001284330. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTCCCCAAACGGCGGCGAGCGCGGGTCGGGTCCCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCC TCGACGCGCTTCCCGGGAGTCGCCATCTACCTGGTCGAGCCTCGCATGGGTCGCAGCCGCCGGGCCTTC CTCACAGGCCTGGCGCGCTCCAAAGGCTTCCGCGTCCTTGACGCCTGCAGCTCCGAAGCGACACATGTT GTGATGGAAGAGACCTCAGCAGAGGAGGCCGTCAGCTGGCAGGAGCGCAGGATGGCAGCTGCTCCCCCG GGTTGCACCCCCCCAGCTCTGCTGGACATAAGCTGGTTAACAGAGAGCCTGGGAGCTGGGCAGCCTGTA CCTGTGGAGTGCCGGCACCGCCTGGAGGTGGCTGGGCCAAGGAAGGGGCCTCTGAGCCCAGCATGGATG CCTGCCTATGCCTGCCAGCGCCCTACGCCCCTCACACACCACAACACTGGCCTCTCCGAGGCTCTGGAG ATACTGGCCGAGGCAGCAGGCTTTGAAGGCAGTGAGGGCCGCCTCCTCACCTTCTGCAGAGCAGCCTCG GTGCTCAAGGCCCTTCCCAGCCCTGTCACAACCCTGAGCCAGCTGCAGGGGCTTCCCCACTTTGGAGAA CACTCCTCTAGGGTTGTCCAGGAGCTGCTGGAGCATGGAGTGTGTGAGGAGGTGGAGAGAGTTCGGCGC TCAGAGAGGGCTCCAGCACCACCAGGACCTGAGCACCCCAGTCCTGCGGTCCGATGTAGATGCCCTGCA GCAGGTGGTGGAGGAAGCTGTGGGGCAGGCCCTGCCTGGGGCCACCGTCACGCTGACCGGCGGCTTCCG CAGGGCCTCATCCTGTACCACCAGCACCAGCACAGCTGCTGTGAGTCCCCTACCCGCCTGGCCCAACAG AGCCACATGGACGCTTTTGAGAGAAGTTTCTGCATTTTCCGCCTACCACAACCTCCAGGGGCTGCTGTG GGGGGATCCACGAGGCCCTGCCCATCCTGGAAGGCCGTGAGAGTGGACTTGGTAGTTGCACCCGTCAGC CAGTTCCCTTTCGCCCTGCTCGGTTGGACTGGCTCCAAGCTTTTCCAGCGGGAGCTGCGCCGCTTCAGC CGGAAGGAGAAGGGCCTGTGGCTGAACAGCCATGGGCTGTTTGACCCGGAGCAGGGAAGCAGCAGTGGC AAGACTCCTAGGTCACGGAAGTCCTGCTTCTGTTGCAGAAGACATTTTTCCAAGCGGCTTCAGAGGAAG ACATCTTCAGACACCTGGGCCTTGAGTACCTTCCTCCAGAGCAGAGAAACGCCTGAGCCTGCCTGTGTC CCCCACTTCCACTCAGGAAATTGGGCTGCCCCCAACCTGGCCACTGAATGTCTCCAGGCAGATATGCTG CCCCCTGACCCCCACCTTCACCCCTCCCCGCCAAGGCCTGGCTCTTCCGGAGGTCAATTGTGCCTGCAG GATCAGTTGAGCCCCTGCTGGTGTGCTGCAGGGTGTGATGAGGTGGGAGCCCTCAGTGCCAGCCTCATC ACTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001284330
Insert Size	1527 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284330.1
RefSeq Size	2638 bp
RefSeq ORF	1527 bp
Locus ID	27434
UniProt ID	Q9NP87
Protein Families	Druggable Genome
Protein Pathways	Non-homologous end-joining
MW	55.1 kDa
Gene Summary	Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has multiple differences in the coding region, compared to variant 1, one of which results in a translational frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is longer than isoform 1.

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