ECSIT (NM_001142465) Human Untagged Clone

CAT#: SC325617

ECSIT (untagged)-Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 3



  "NM_001142465" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


ECSIT rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms SITPEC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC325617 representing NM_001142465.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGCTGGGTCCAGGCCACCCTACTGGCCCGAGGCCTCTGTAGGGCCTGGGGAGGCACCTGCGGGGCC
GCCCTCACAGGAACCTCCATCTCTCAGGTTCCTTTGCCCAAAGACTCAACAGGTGCAGCAGATCCCCCC
CAGCCCCACATCGTAGGAATCCAGAGTCCCGATCAGCAGGCCGCCCTGGCCCGCCACAATCCAGCCCGG
CCTGTCTTTGTTGAGGGCCCCTTCTCCCTGTGGCTCCGCAACAAGTGTGTGTATTACCACATCCTCAGA
GCTGACTTGCTGCCCCCGGAGGAGAGGGAAGTGGAAGAGACGCCGGAGGAGTGGAACCTCTACTACCCG
ATGCAGCTGGACCTGGAGTATGTGAGGAGTGGCTGGGACAACTACGAGTTTGACATCAATGAAGTGGAG
GAAGGCCCTGTCTTCGCCATGTGCATGGCGGGTGCTCATGACCAGGCGACGATGGCTAAGTGGATCCAG
GGCCTGCAGGAGACCAACCCAACCCTGGCCCAGATCCCCGTGGTCTTCCGCCTCGCCGGGTCCACCCGG
GAGCTCCAGACATCCTCTGCAGGGCTGGAGGAGCCGCCCCTGCCCGAGGACCACCAGGAAGAAGACGAC
AACCTGCAGCGACAGCAGCAGGGCCAGAGCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001142465
Insert Size 654 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001142465.2
RefSeq Size 1068 bp
RefSeq ORF 654 bp
Locus ID 51295
UniProt ID Q9BQ95
Protein Pathways MAPK signaling pathway
MW 24.2 kDa
Gene Summary Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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