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CLK3 (NM_003992) Human Untagged Clone
CAT＃: SC323354
CLK3 (untagged)-Kinase deficient mutant (K186M) of Human CDC-like kinase 3 (CLK3), transcript variant 2
CNY 3,656.00
CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PHCLK3; PHCLK3/152 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003992, the custom clone sequence may differ by one or more nucleotides
ATGCATCACTGTAAGCGATACCGCTCCCCTGAACCAGACCCGTACCTGAGCTACCGATGGAAGAGGAGGA GGTCCTACAGTCGGGAACATGAAGGGAGACTGCGATACCCGTCCCGAAGGGAGCCTCCCCCACGAAGATC TCGGTCCAGAAGCCATGACCGCCTGCCCTACCAGAGGAGGTACCGGGAGCGCCGTGACAGCGATACATAC CGGTGTGAAGAGCGGAGCCCATCCTTTGGAGAGGACTACTATGGACCTTCACGTTCTCGTCATCGTCGGC GATCGCGGGAGAGGGGGCCATACCGGACCCGCAAGCATGCCCACCACTGCCACAAACGCCGCACCAGGTC TTGTAGCAGCGCCTCCTCGAGAAGCCAACAGAGCAGTAAGCGCAGCAGCCGGAGTGTGGAAGATGACAAG GAGGGTCACCTGGTGTGCCGGATCGGCGATTGGCTCCAAGAGCGATATGAGATTGTGGGGAACCTGGGTG AAGGCACCTTTGGCAAGGTGGTGGAGTGCTTGGACCATGCCAGAGGGAAGTCTCAGGTTGCCCTGAAGAT CATCCGCAACGTGGGCAAGTACCGGGAGGCTGCCCGGCTAGAAATCAACGTGCTCAAAAAAATCAAGGAG AAGGACAAAGAAAACAAGTTCCTGTGTGTCTTGATGTCTGACTGGTTCAACTTCCACGGTCACATGTGCA TCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCTGAAGGAGAATAACTTCCAGCCTTACCCCCT ACCACATGTCCGGCACATGGCCTACCAGCTCTGCCACGCCCTTAGATTTCTGCATGAGAATCAGCTGACC CATACAGACTTGAAACCAGAGAACATCCTGTTTGTGAATTCTGAGTTTGAAACCCTCTACAATGAGCACA AGAGCTGTGAGGAGAAGTCAGTGAAGAACACCAGCATCCGAGTGGCTGACTTTGGCAGTGCCACATTTGA CCATGAGCACCACACCACCATTGTGGCCACCCGTCACTATCGCCCGCCTGAGGTGATCCTTGAGCTGGGC TGGGCACAGCCCTGTGACGTCTGGAGCATTGGCTGCATTCTCTTTGAGTACTACCGGGGCTTCACACTCT TCCAGACCCACGAAAACCGAGAGCACCTGGTGATGATGGAGAAGATCCTAGGGCCCATCCCATCACACAT GATCCACCGTACCAGGAAGCAGAAATATTTCTACAAAGGGGGCCTAGTTTGGGATGAGAACAGCTCTGAC GGCCGGTATGTGAAGGAGAACTGCAAACCTCTGAAGAGTTACATGCTCCAAGACTCCCTGGAGCACGTGC AGCTGTTTGACCTGATGAGGAGGATGTTAGAATTTGACCCTGCCCAGCGCATCACACTGGCCGAGGCCCT GCTGCACCCCTTCTTTGCTGGCCTGACCCCTGAGGAGCGGTCCTTCCACACCAGCCGCAACCCAAGCAGA TGA >OriGene 5' read for mutant NM_003992 unedited
CCGCCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGATGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGTCTCCACCT CTGCCTTTGACTGACACCTCAATCTGTCAATCGGAACCGCCTACCATGCGATTGGTCGGATGGCGTTTCG GGGGGCGGTGCATGCAGAGAAGCCAACAGAGCAGTAAGCGCAGCAGCCGGAGTGTGGAAGATGACAAGGA GGGTCACCTGGTGTGCCGGATCGGCGATTGGCTCCAAGAGCGATGTACAGGCCACCCTTTCGTAAGACTG TTAGCCTCTTCTACTTTCTACCCCCCCTTGTTAAAACGAAGAACCTCTTCCCTGGCCCTGGAAGGGGCCT CCTAGTCGCCCGGTGGTGGCCTTACCCGGGGCCCCCAGTAATGGGCCTGATGGAACACAAACCCTAGCAA CCTTCAATTTTTTAAAAGGGTACCATGGGAAAAAAAAACCTTTTTTGGTTTTTTGAAAATTTAAATTGCG GGGACTTGGGCGGGGATGGGCAACCCTGGGCTTAAAAATTCAAGGCCCAATTTTTTTTTCTTCCCTAAAG AATTTGGGGGGAACCCGGGGTAAAGGGCACCTTTGGCAGGGGGGGGAGAGTTTTGACCCACCCCAAAGGG AATCTCTAGGTGTCCCCAGGAGAATCACGCAAGTTGGCGCATATTCGCGGGGGGGCCCCGCGTGTAAAAC TCCCTGCTGCTAAAAATCACGAGGAGGAAGCACAAAACAATTTTTTTTGTGGAGAGATCATTGAGAAAAC ACCCTCCTCTGGGTGGCTTTTGTGTGGCCCCCTTTTATCACAGAGCACACACACTTGTGTGCACCGTCCG TATCTCCCTGAGTGATACTCTAGCTGGCGGGCGCAACAGGAGCTCAGTGATAGCGAGCTCTTCGATATAA CAGCCGTCATCCGTCGTACAGCGCAGCACGTAGCCGACGTCG >SC323354 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSACTGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGTCTCCACCTCTGCCTT TGACTGACACCTCAATCTGTCAATCGGAACCGCCTACCATGCGATTGGTCGGATGGCGTTTCGGGGGGCG GTGCATGCAGAGAAGCCAACAGAGCAGTAAGCGCAGCAGCCGGAG |
| Restriction Sites | Please inquire |
| ACCN | NM_003992 |
| Insert Size | 1870 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003992.1, NP_003983.1 |
| RefSeq Size | 1762 bp |
| RefSeq ORF | 1473 bp |
| Locus ID | 1198 |
| UniProt ID | P49761 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200489 | CLK3 (Myc-DDK-tagged)-Human CDC-like kinase 3 (CLK3), transcript variant 2 |
CNY 4,850.00 |
|
| RC200489L3 | Lenti-ORF clone of CLK3 (Myc-DDK-tagged)-Human CDC-like kinase 3 (CLK3), transcript variant 2 |
CNY 6,750.00 |
|
| RC200489L4 | Lenti-ORF clone of CLK3 (mGFP-tagged)-Human CDC-like kinase 3 (CLK3), transcript variant 2 |
CNY 6,750.00 |
|
| RG200489 | CLK3 (tGFP-tagged) - Human CDC-like kinase 3 (CLK3), transcript variant 2 |
CNY 5,320.00 |
|
| SC109075 | CLK3 (untagged)-Human CDC-like kinase 3 (CLK3), transcript variant 2 |
CNY 2,950.00 |
|
| SC320215 | CLK3 (untagged)-Human CDC-like kinase 3 (CLK3), transcript variant 2 |
CNY 2,950.00 |
|
| SC323407 | CLK3 (untagged)-Kinase deficient mutant (K186M) of Human CDC-like kinase 3 (CLK3), transcript variant 2 |
CNY 3,656.00 |
