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Perforin (PRF1) (NM_001083116) Human Untagged Clone
CAT＃: SC322683
PRF1 (untagged)-Human perforin 1 (pore forming protein) (PRF1), transcript variant 2
CNY 4,144.00
CNY 8,840.00
Product images
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HPLH2; P1; PFP |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for SC322683
GGCTGGTGCAAGGAGCCACAGTGGGCTGCCTGGGGGGCTGATGCCACCATTCCAGGAGCC
TCGGTGAAGAGAGGATATCCATCTGTGTAGCCGCTTCTCTATACGGGATTCCAGCTCCAT GGCAGCCCGTCTGCTCCTCCTGGGCATCCTTCTCCTGCTGCTGCCCCTGCCCGTCCCTGC CCCGTGCCACACAGCCGCACGCTCAGAGTGCAAGCGCAGCCACAAGTTCGTGCCTGGTGC ATGGCTGGCCGGGGAGGGTGTGGACGTGACCAGCCTCCGCCGCTCGGGCTCCTTCCCAGT GGACACACAAAGGTTCCTGCGGCCCGACGGCACCTGCACCCTCTGTGAAAATGCCCTACA GGAGGGCACCCTCCAGCGCCTGCCTCTGGCGCTCACCAACTGGCGGGCCCAGGGCTCTGG CTGCCAGCGCCATGTAACCAGGGCCAAAGTCAGCTCCACTGAAGCTGTGGCCCGGGATGC GGCTCGTAGCATCCGCAACGACTGGAAGGTCGGGCTGGACGTGACTCCTAAGCCCACCAG CAATGTGCATGTGTCTGTGGCCGGCTCACACTCACAGGCAGCCAACTTTGCAGCCCAGAA GACCCACCAGGACCAGTACAGCTTCAGCACTGACACGGTGGAGTGCCGCTTCTACAGTTT CCATGTGGTACACACTCCCCCGCTGCACCCTGACTTCAAGAGGGCCCTCGGGGACCTGCC CCACCACTTCAACGCCTCCACCCAGCCCGCCTACCTCAGGCTTATCTCCAACTACGGCAC CCACTTCATCCGGGCTGTGGAGCTGGGTGGCCGCATATCGGCCCTCACTGCCCTGCGCAC CTGCGAGCTGGCCCTGGAAGGGCTCACGGACAACGAGGTGGAGGACTGCCTGACTGTCGA GGCCCAGGTCAACATAGGCATCCACGGCAGCATCTCTGCCGAAGCCAAGGCCTGTGAGGA GAAGAAGAAGAAGCACAAGATGACGGCCTCCTTCCACCAAACCTACCGGGAGCGCCATTC GGAAGTGGTTGGCGGCCATCACACCTCCATTAACGACCTGCTGTTCGGGATCCAGGCCGG GCCCGAGCAGTACTCAGCCTGGGTAAACTCGCTGCCCGGCAGCCCTGGCCTGGTGGACTA CACCCTGGAACCCCTGCACGTGCTGCTGGACAGCCAGGACCCGCGGCGGGAGGCACTGAG GAGGGCCCTGAGTCAGTACCTGACGGACAGGGCTCGCTGGAGGGACTGCAGCCGGCCGTG CCCACCAGGGCGGCAGAAGAGCCCCCGAGACCCATGCCAGTGTGTGTGCCATGGCTCAGC GGTCACCACCCAGGACTGCTGCCCTCGGCAGAGGGGCCTGGCCCAGCTGGAGGTGACCTT CATCCAAGCATGGGGCCTGTGGGGGGACTGGTTCACTGCCACGGATGCCTATGTGAAGCT CTTCTTTGGTGGCCAGGAGCTGAGGACGAGCACCGTGTGGGACAATAACAACCCCATCTG GTCAGTGCGGCTGGATTTTGGGGATGTGCTCCTGGCCACAGGGGGGCCCCTGAGGTTGCA GGTCTGGGATCAGGACTCTGGCAGGGACGATGACCTCCTTGGCACCTGTGATCAGGCTCC CAAGTCTGGTTCCCATGAGGTGAGATGCAACCTGAATCATGGCCACCTAAAATTCCGCTA TCATGCCAGGTGCTTGCCCCACCTGGGAGGAGGCACCTGCCTGGACTATGTCCCCCAAAT GCTTCTGGGGGAGCCTCCAGGAAACCGGAGTGGGGCCGTGTGGTGAGAACAGTGAGCTTG GAAAGGACCAGTATGCTTGGACTGAAGGGGTTCTCACAGTGGGAGCCAGGGCTGTCTTCG TATTCCCATTAGACCAAGCTTATCCAACCCGAGGCCCGCATGCGGCCCAGGATGGCTTTG AATGCGGCCCAACGCAAATTCGCAAACTTTCTTAAAACATTATGAGTTTCTTTTTGCTAT TTTTTTTTTTTTTTTTAGCTCATCGGCTATCGTTAGTGCTAGTGGATTTTACATGTGGCC CAACACAATTCTTCTTCCAACGTGGCCCAGAGAAGCCAAAAGATTGGATACGCATCAGAC AGATGGAAAAGGGAGATTCAGACTGTTTTTCAGGGAGGTGGCTGGGTTTACACGCTAATC CCGATTCACCCTGTCCAAACTGCCTAAGCCCTCCGCCATTCTCAAGCCCTGCAGTCACAG CTACACAGATCACAGCTTCAGCCAGGAGCTGGGCAGAAGGCCAAGAGGCTGTTCCCACCA GGCTGCTCAGGGCTGGTCTTTTAGGACCCTTCCCTTGAGCCCTCTATGGTGTGGCAAAGC CTTCATTGCCTTAACTGGAGCCCCATCAGCTCCAGCTGCTCTGTCTTCTTTGCCCACAAT GCTTTGCCCCTGAGACAAATGGAGGCCTGTCCTGACCTGTCTCACCATGTACATAGCTTG ATAAAGGGCCAATAAATATGATGTTATGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001083116 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001083116.1, NP_001076585.1 |
| RefSeq Size | 2555 bp |
| RefSeq ORF | 1668 bp |
| Locus ID | 5551 |
| UniProt ID | P14222 |
| Protein Families | Druggable Genome |
| Protein Pathways | Allograft rejection, Autoimmune thyroid disease, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis |
| Gene Summary | This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208940 | PRF1 (Myc-DDK-tagged)-Human perforin 1 (pore forming protein) (PRF1), transcript variant 2 |
CNY 4,136.00 |
|
| RC208940L3 | Lenti-ORF clone of PRF1 (Myc-DDK-tagged)-Human perforin 1 (pore forming protein) (PRF1), transcript variant 2 |
CNY 6,460.00 |
|
| RC208940L4 | Lenti-ORF clone of PRF1 (mGFP-tagged)-Human perforin 1 (pore forming protein) (PRF1), transcript variant 2 |
CNY 6,460.00 |
|
| RG208940 | PRF1 (tGFP-tagged) - Human perforin 1 (pore forming protein) (PRF1), transcript variant 2 |
CNY 5,040.00 |
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