QRICH1 (AK074313) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK074313, the custom clone sequence may differ by one or more nucleotides ATGAATAATTCCCTAGAGAACACCATCTCCTTTGAAGAGTACATCCGAGTAAAGGCACGG TCTGTCCCGCAACACAGGATGAAGGAATTTCTGGACTCACTGGCCTCTAAGGGGCCAGAA GCCCTTCAGGAGTTCCAGCAGACAGCCACCACTACCATGGTGTACCAACAGGGTGGGAAC TGCATATACACAGACAGCACTGAAGTGGCTGGGTCTTTGCTTGAACTTGCCTGTCCAGTC ACCACCAGTGTTCAGCCACAAACCCAGCAAGAACAGCAGATCCAGGTTCAGCAGCCGCAG CAGGTTCAGGTCCAGGTGCAGGTACAGCAGTCTCCGCAACAGGTCTCGGCTCAGCTCTCC CCACAACTCACCGTTCACCAGCCTACTGAGCAACCCATCCAGGTCCAGGTGCAGATCCAA GGCCAGGCACCACAGTCAGCAGCCCCCTCCATTCAGACCCCGTCTCTGCAGAGTCCCAGT CCCTCGCAGCTGCAAGCAGCTCAGATCCAGGTGCAGCACGTGCAAGCAGCCCAGCAGATC CAGGCTGCAGAAATCCCGGAGGAGCACATCCCACATCAGCAAATCCAGGCTCAGCTGGTG GCTGGCCAGTCTCTTGCTGGTGGTCAGCAGATCCAAATCCAGACCGTGGGTGCCCTTTCC CCACCACCATCCCAGCAGGGCTCACCCCGGGAAGGGGAGCGGCGGGTTGGCACGGCCAGT GTCCTCCAACCAGTGAAGAAGCGCAAAGTGGAGATGCCCATCACTGTGTCCTACGCCATC TCAGGGCAGCCGGTGGCCACCGTGCTGGCCATTCCACAGGGCCAGCAGCAGAGTTATGTG TCTTTGAGGCCAGACTTACTGACAGTAGACAGTGCCCACCTGTACAGTGCCACTGGGACC ATTACTAGCCCTACAGGAGAAACCTGGACCATCCCTGTTTATTCTGCCCAGCCCCGGGGG GACCCTCAGCAGCAGAGCATTACCCACATTGCCATTCCCCAGGAAGCCTACAACGCAGTT CACGTCAGTGGCTCACCCACAGCCCTGGCAGCTGTTAAGCTGGAGGATGACAAGGAGAAG ATGGTGGGCACCACATCTGTAGTGAAAAACTCCCATGAAGAGGTAGTGCAGACCCTTGCA AACTCTCTCTTTCCAGCACAGTTCATGAATGGCAACATCCACATTCCAGTGGCTGTGCAG GCTGTGGCAGGCACGTACCAGAATACGGCTCAAACTGTCCATATATGGGACCCCCAACAG CAGCCGCAGCAGCAAACTCCCCAGGAACAGACACCACCACCACAGCAGCAGCAGCAGCAA CTCCAAGTTACTTGTTCAGCTCAAACTGTCCAGGTTGCTGAAGTTGAACCACAGTCACAG CCACAGCCTTCCCCAGAACTTCTGCTTCCAAATTCTTTGAAGCCAGAAGAAGGGCTTGAA GTATGGAAAAACTGGGCCCAGACCAAGAATGCTGAACTAGAGAAGGATGCTCAGAACAGA TTGGCACCCATTGGGAGGCGCCAACTGCTGCGATTCCAGGAAGATCTCATCTCCTCTGCT GTGGCAGAGTTGAATTATGGGCTCTGTCTAATGACACGGGAAGCTCGAAATGGAGAAGGT GAACCCTATGACCCAGATGTGCTCTACTATATTTTCCTGTGTATTCAAAAGTATCTTTTT GAAAATGGAAGGGTAGATGACATTTTCTCCGATCTTTATTATGTTCGGTTCACGGAGTGG CTACATGAAGTTCTGAAGGATGTTCAGCCCCGGGTCACTCCACTTGGCTATGTCTTGCCC AGCCACGTGACTGAGGAGATGCTATGGGAGTGCAAGCAGCTTGGGGCTCACTCCCCCTCC ACCTTGCTGACCACCCTCATGTTCTTTAATACCAAGTACTTCCTATTGAAGACAGTGGAC CAGCACATGAAGCTGGCCTTCTCCAAGGTCTTGCGACAGACAAAGAAGAACCCCTCTAAT CCCAAGGATAAAAGCACGAGTATCCGGTACTTGAAGGCCCTTGGAATACACCAGACTGGC CAGAAAGTTACAGATGACATGTATGCAGAACAGACGGAAAATCCAGAGAATCCATTGAGA TGTCCCATCAAGCTCTATGATTTCTACCTCTTCAAATGCCCCCAGAGTGTGAAAGGCCGG AATGACACCTTTTACCTGACACCTGAGCCAGTGGTGGCCCCCAACAGCCCAATCTGGTAC TCAGTCCAGCCTATCAGCAGAGAGCAGATGGGACAAATGCTGACGCGGATCCTGGTGATA AGAGAAATTCAGGAGGCCATCGCAGTGGCCAATGCAAGCACTATGCAC
Restriction Sites	Please inquire
ACCN	AK074313
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK074313.1, BAB85047.1
RefSeq Size	3101 bp
RefSeq ORF	2331 bp
Locus ID	54870
Protein Families	Druggable Genome
Gene Summary	Transcriptional regulator that acts as a mediator of the integrated stress response (ISR) through transcriptional control of protein homeostasis under conditions of ER stress (PubMed:33384352). Controls the outcome of the unfolded protein response (UPR) which is an ER-stress response pathway (PubMed:33384352). ER stress induces QRICH1 translation by a ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced QRICH1 regulates a transcriptional program associated with protein translation, protein secretion-mediated proteotoxicity and cell death during the terminal UPR (PubMed:33384352). May cooperate with ATF4 transcription factor signaling to regulate ER homeostasis which is critical for cell viability (PubMed:33384352). Upregulates CASP3/caspase-3 activity in epithelial cells under ER stress. Central regulator of proteotoxicity associated with ER stress-mediated inflammatory diseases in the intestines and liver (PubMed:33384352). Involved in chondrocyte hypertrophy, a process required for normal longitudinal bone growth (PubMed:30281152).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.