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P4HA2 (NM_004199) Human Untagged Clone
CAT＃: SC310290
P4HA2 (untagged)-Human prolyl 4-hydroxylase, alpha polypeptide II (P4HA2), transcript variant 1
CNY 5,984.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MYP25 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC310290 representing NM_004199.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAACTCTGGGTGTCTGCATTGCTGATGGCCTGGTTTGGTGTCCTGAGCTGTGTGCAGGCCGAATTC TTCACCTCTATTGGGCACATGACTGACCTGATTTATGCAGAGAAAGAGCTGGTGCAGTCTCTGAAAGAG TACATCCTTGTGGAGGAAGCCAAGCTTTCCAAGATTAAGAGCTGGGCCAACAAAATGGAAGCCTTGACT AGCAAGTCAGCTGCTGATGCTGAGGGCTACCTGGCTCACCCTGTGAATGCCTACAAACTGGTGAAGCGG CTAAACACAGACTGGCCTGCGCTGGAGGACCTTGTCCTGCAGGACTCAGCTGCAGGTTTTATCGCCAAC CTCTCTGTGCAGCGGCAGTTCTTCCCCACTGATGAGGACGAGATAGGAGCTGCCAAAGCCCTGATGAGA CTTCAGGACACATACAGGCTGGACCCAGGCACAATTTCCAGAGGGGAACTTCCAGGAACCAAGTACCAG GCAATGCTGAGTGTGGATGACTGCTTTGGGATGGGCCGCTCGGCCTACAATGAAGGGGACTATTATCAT ACGGTGTTGTGGATGGAGCAGGTGCTAAAGCAGCTTGATGCCGGGGAGGAGGCCACCACAACCAAGTCA CAGGTGCTGGACTACCTCAGCTATGCTGTCTTCCAGTTGGGTGATCTGCACCGTGCCCTGGAGCTCACC CGCCGCCTGCTCTCCCTTGACCCAAGCCACGAACGAGCTGGAGGGAATCTGCGGTACTTTGAGCAGTTA TTGGAGGAAGAGAGAGAAAAAACGTTAACAAATCAGACAGAAGCTGAGCTAGCAACCCCAGAAGGCATC TATGAGAGGCCTGTGGACTACCTGCCTGAGAGGGATGTTTACGAGAGCCTCTGTCGTGGGGAGGGTGTC AAACTGACACCCCGTAGACAGAAGAGGCTTTTCTGTAGGTACCACCATGGCAACAGGGCCCCACAGCTG CTCATTGCCCCCTTCAAAGAGGAGGACGAGTGGGACAGCCCGCACATCGTCAGGTACTACGATGTCATG TCTGATGAGGAAATCGAGAGGATCAAGGAGATCGCAAAACCTAAACTTGCACGAGCCACCGTTCGTGAT CCCAAGACAGGAGTCCTCACTGTCGCCAGCTACCGGGTTTCCAAAAGCTCCTGGCTAGAGGAAGATGAT GACCCTGTTGTGGCCCGAGTAAATCGTCGGATGCAGCATATCACAGGGTTAACAGTAAAGACTGCAGAA TTGTTACAGGTTGCAAATTATGGAGTGGGAGGACAGTATGAACCGCACTTCGACTTCTCTAGGAATGAT GAGCGAGATACTTTCAAGCATTTAGGGACGGGGAATCGTGTGGCTACTTTCTTAAACTACATGAGTGAT GTAGAAGCTGGTGGTGCCACCGTCTTCCCTGATCTGGGGGCTGCAATTTGGCCTAAGAAGGGTACAGCT GTGTTCTGGTACAACCTCTTGCGGAGCGGGGAAGGTGACTACCGAACAAGACATGCTGCCTGCCCTGTG CTTGTGGGCTGCAAGTGGGTCTCCAATAAGTGGTTCCATGAACGAGGACAGGAGTTCTTGAGACCTTGT GGATCAACAGAAGTTGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_004199 |
| Insert Size | 1608 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004199.2 |
| RefSeq Size | 2588 bp |
| RefSeq ORF | 1608 bp |
| Locus ID | 8974 |
| UniProt ID | O15460 |
| Domains | 2OG-FeII_Oxy, P4Hc |
| Protein Families | Druggable Genome |
| Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
| MW | 60.9 kDa |
| Gene Summary | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1), also known as IIb, encodes the longest isoform (1). Variants 1, 5, and 6 all encode the same isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219031 | P4HA2 (Myc-DDK-tagged)-Human prolyl 4-hydroxylase, alpha polypeptide II (P4HA2), transcript variant 1 |
CNY 5,976.00 |
|
| RC219031L3 | Lenti-ORF clone of P4HA2 (Myc-DDK-tagged)-Human prolyl 4-hydroxylase, alpha polypeptide II (P4HA2), transcript variant 1 |
CNY 6,460.00 |
|
| RC219031L4 | Lenti-ORF clone of P4HA2 (mGFP-tagged)-Human prolyl 4-hydroxylase, alpha polypeptide II (P4HA2), transcript variant 1 |
CNY 6,460.00 |
|
| RG219031 | P4HA2 (tGFP-tagged) - Human prolyl 4-hydroxylase, alpha polypeptide II (P4HA2), transcript variant 1 |
CNY 5,040.00 |
