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HIF-1 alpha (HIF1A) (NM_181054) Human Untagged Clone
CAT＃: SC127304
HIF1A (untagged)-Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2
CNY 8,088.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127304 sequence for NM_181054 edited (data generated by NextGen Sequencing)
ATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAAAGATAAGTTCTGAACGTCGAAAAGAA AAGTCTCGAGATGCAGCCAGATCTCGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGCTT GCTCATCAGTTGCCACTTCCACATAATGTGAGTTCGCATCTTGATAAGGCCTCTGTGATG AGGCTTACCATCAGCTATTTGCGTGTGAGGAAACTTCTGGATGCTGGTGATTTGGATATT GAAGATGACATGAAAGCACAGATGAATTGCTTTTATTTGAAAGCCTTGGATGGTTTTGTT ATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAATGTGAACAAATACATG GGATTAACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACTCATCCATGTGAC CATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCTTGTGAAAAAGGGTAAAGAA CAAAACACACAGCGAAGCTTTTTTCTCAGAATGAAGTGTACCCTAACTAGCCGAGGAAGA ACTATGAACATAAAGTCTGCAACATGGAAGGTATTGCACTGCACAGGCCACATTCACGTA TATGATACCAACAGTAACCAACCTCAGTGTGGGTATAAGAAACCACCTATGACCTGCTTG GTGCTGATTTGTGAACCCATTCCTCACCCATCAAATATTGAAATTCCTTTAGATAGCAAG ACTTTCCTCAGTCGACACAGCCTGGATATGAAATTTTCTTATTGTGATGAAAGAATTACC GAATTGATGGGATATGAGCCAGAAGAACTTTTAGGCCGCTCAATTTATGAATATTATCAT GCTTTGGACTCTGATCATCTGACCAAAACTCATCATGATATGTTTACTAAAGGACAAGTC ACCACAGGACAGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGGGTTGAAACTCAA GCAACTGTCATATATAACACCAAGAATTCTCAACCACAGTGCATTGTATGTGTGAATTAC GTTGTGAGTGGTATTATTCAGCACGACTTGATTTTCTCCCTTCAACAAACAGAATGTGTC CTTAAACCGGTTGAATCTTCAGATATGAAAATGACTCAGCTATTCACCAAAGTTGAATCA GAAGATACAAGTAGCCTCTTTGACAAACTTAAGAAGGAACCTGATGCTTTAACTTTGCTG GCCCCAGCCGCTGGAGACACAATCATATCTTTAGATTTTGGCAGCAACGACACAGAAACT GATGACCAGCAACTTGAGGAAGTACCATTATATAATGATGTAATGCTCCCCTCACCCAAC GAAAAATTACAGAATATAAATTTGGCAATGTCTCCATTACCCACCGCTGAAACGCCAAAG CCACTTCGAAGTAGTGCTGACCCTGCACTCAATCAAGAAGTTGCATTAAAATTAGAACCA AATCCAGAGTCACTGGAACTTTCTTTTACCATGCCCCAGATTCAGGATCAGACACCTAGT CCTTCCGATGGAAGCACTAGACAAAGTTCACCTGAGCCTAATAGTCCCAGTGAATATTGT TTTTATGTGGATAGTGATATGGTCAATGAATTCAAGTTGGAATTGGTAGAAAAACTTTTT GCTGAAGACACAGAAGCAAAGAACCCATTTTCTACTCAGGACACAGATTTAGACTTGGAG ATGTTAGCTCCCTATATCCCAATGGATGATGACTTCCAGTTACGTTCCTTCGATCAGTTG TCACCATTAGAAAGCAGTTCCGCAAGCCCTGAAAGCGCAAGTCCTCAAAGCACAGTTACA GTATTCCAGCAGACTCAAATACAAGAACCTACTGCTAATGCCACCACTACCACTGCCACC ACTGATGAATTAAAAACAGTGACAAAAGACCGTATGGAAGACATTAAAATATTGATTGCA TCTCCATCTCCTACCCACATACATAAAGAAACTACTAGTGCCACATCATCACCATATAGA GATACTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAAAGGAGTCATAGAACAGACA GAAAAATCTCATCCAAGAAGCCCTAACGTGTTATCTGTCGCTTTGAGTCAAAGAACTACA GTTCCTGAGGAAGAACTAAATCCAAAGATACTAGCTTTGCAGAATGCTCAGAGAAAGCGA AAAATGGAACATGATGGTTCACTTTTTCAAGCAGTAGGAATTATTTAG Clone variation with respect to NM_181054.2 >OriGene 5' read for NM_181054 unedited
TGTATACGACCTCACTATAGGGCGGCCGCGAATTCGCACGAGGCACCTCTGGACTTGCCT TTCCTTCTCTTCTCCGCGTGTGGAGGGAGCCAGCGCTTAGGCCGGAGCGAGCCTGGGGGC CGCCCGCCGTGAAGACATCGCGGGGACCGATTCACCATGGAGGGCGCCGGCGGCGCGAAC GACAAGAAAAAGATAAGTTCTGAACGTCGAAAAGAAAAGTCTCGAGATGCAGCCAGATCT CGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCCACAT AATGTGAGTTCGCATCTTGATAAGGCCTCTGTGATGAGGCTTACCATCAGCTATTTGCGT GTGAGGAAACTTCTGGATGCTGGTGATTTGGATATTGAAGATGACATGAAAGCACAGATG AATTGCTTTTATTTGAAAGCCTTGGATGGTTTTGTTATGGTTCTCACAGATGATGGTGAC ATGATTTACATTTCTGATAATGTGAACAAATACATGGGATTAACTCAGTTTGAACTAACT GGACACAGTGTGTTTGATTTTACTCATCCATGTGACCATGAGGAAATGAGAGAAATGCTT ACCACAGAAATGGCCTTGTGAAAAAGGGTAAAGAACAAAACACACAGCGAAGCTTTTTTC TCAAATGAAGTGTACCCTAACTAGCCGAGGAAGAACTATGAACATAAAGTCTGCACATGG GAAGGTATTGCACTGCACAGGCCCATTCACGTATATGATACCACAGTAACCAACCTCAGT GTGGGGATAGAAACCACTATGACCTGGCTGGTGCTGATTTGGGACCATTTCTCACCTCAA TATGGAAANTCTTAATAGCAGACTTTCTCATCGAACACCTGAATGAAATTNCTTATGGAT GAAGATACCGATGATGGNAATAGCCAAGAATTTTAGCCGCCATTTGAAATATAGGCTGAA CA >OriGene 3' read for NM_181054 unedited
AATCTAGAGTCGAGTTTTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCAAAAAATGT TTATTTGATGTAACAAAACAATACAGTTAGTGTTAGATCCAACCACAAAGAGCAAAAGGA ATGAAAAATTTGTCCATTGTCCATAGAAAAAACAAGATTTTTACTGGGACAACTATATAT TCCTAAAATAATGCTTCTAAAATTACTCAATTATTGAAATCTACATGAAAAAAAGGATGT TAATAGCGACAAAGTGCATAAAATCAAACATTGTATTTTGAGCAAATTAACATACTAGGC AATTTTGCTAAAAATGCATGATTTTTTTTTTCTTGTTAACAGTCTGCTCAAAATATCTTT ATACCAACAGGGTAGGCAAAACATTTAGGTTTAATATCAGTTACACAATATTAGCATAAA CTTCCACAACTACATAGGGTATTGTTTTCTTTTGAGCTGGCAAAGGGACTATAAAAACAT CAGATGATTTCTCTGAATTGAAAATTTTATCCAAATAAATGCCACATACCTTCTAGATAT ATGCATATCTTTCTATATTATGTAAATGGCTTTACCCATTTAAATAATAAACCATACAGC ATTTAAGAATCATTATTATATGATTAACAATGTCATGTTCCAGGTTTAACAATTTCATAG GCCAAAAAAAATTTCTTCTTAAAAACTAGTTTTATAAACGCAGAATTATTCCATGAGTAC TGCTGGTATTTTTTAAGAAATATATTGTGCAATTGTGGCTACCACGTACTGCTGGCAAAG CATATTATTTATGTAAAATGTGAAAAAAAGGTGTAAAATTTTTCACTGCTATGATATGAG GAAGGTACCGGCTTCTACAAAATTTATGGGCATCTCTAAAATATTCGAAAAGGATAACTC CTAGCCAATTATANTAAAAGGGCATTTTAAATGAGCTCTGATGCATGGTTATACAACATG AAATGACTGTTGGGATCACTTAGGACTCTAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_181054 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181054.1, NP_851397.1 |
| RefSeq Size | 3812 bp |
| RefSeq ORF | 2208 bp |
| Locus ID | 3091 |
| UniProt ID | Q16665 |
| Protein Families | Transcription Factors |
| Protein Pathways | mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma |
| Gene Summary | This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011] Transcript Variant: This variant (2) lacks the penultimate coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mechanism of hypoxia-inducible factor 1{alpha}-mediated Mcl1 regulation in Helicobacter pylori-infected human gastric epithelium
,Asima Bhattacharyya, Ranajoy Chattopadhyay, Emily H. Hall, Semret T. Mebrahtu, Peter B. Ernst, and Sheila E. Crowe,
Am J Physiol Gastrointest Liver Physiol, Nov 2010; 299: G1177 - G1186
[HIF1A]
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Epidermal growth factor receptor regulates MET levels and invasiveness through hypoxia-inducible factor-1a in non-small cell lung cancer cells
,L Xu, M B Nilsson, P Saintigny, T Cascone, M H Herynk, Z Du, P G Nikolinakos, Y Yang, L Prudkin, D Liu, et al.,
Oncogene (15 February 2010) doi:10.1038/onc.2010.16 Original Article
[HIF1A]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218966 | HIF1A (Myc-DDK-tagged)-Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2 |
CNY 8,072.00 |
|
| RC218966L1 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2, Myc-DDK-tagged |
CNY 10,472.00 |
|
| RC218966L2 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2, mGFP tagged |
CNY 10,472.00 |
|
| RC218966L3 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC218966L4 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| RG218966 | HIF1A (tGFP-tagged) - Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2 |
CNY 9,672.00 |
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