PIGC (BC008179) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC008179 edited TCTCGGTCGTGCACCCCCCTAAAGAGCTTGGTGCAGGAATTTAGCTGTGTTGTCCTGACC CTCAGGACAAACTCATCTAATGCAGGCTCAGCTTGTACTGAAGGCTTTGTAAACAAGGAA AGTTCTGTAGGGTATCAGCATGGCATTTCCAGAGAGTTCTTAATCTACAGATTTATCAAG AACCAGAACCCCATGGACGTCCCTGAGGGAGATTGAGCCAACCTGGATTTGAGGAAAGAA TGAGATAATGTGTGCTCAACGTGTAACAGACACCCCAGAAGTCAAGTGGCAGAAGGTTTT ATATGAGCGGCAGCCGTTCCCTGATAACTATGTTGACCAGAGGTTCCTGGAAGAACTCCG GAAAAACATCTATGCCCGGAAGTACCAGTACTGGGCTGTGGTATTTGAGTCCAGTGTGGT GATACAGCAGCTGTGCAGTGTCTGTGTTTTTGTAGTGATCTGGTGGTACATGGATGAGGG TCTTCTGGCTCCCCAGTGGCTTTTTGGGACCGGACTGGCATCTTCCTTGGTTGGGTATGT TTTATTCGATCTTATCGATGGAGGTGATGGACGGAAGAAGAGTGGGCGGACCCGGTGGGC CGACCTGAAGAGTACTCTGGTCTTTATCACTTTCACTTACGGTTTTTCACCTGTGCTAAA GACCCTGACGGAGTCTGTCAGTACAGACACTATCTACGCCATGGCAGTCTTTATGCTGTT AGGCCATCTCATCTTCTTTGATTACGGTGCCAATGCTGCTATTGTATCCAGCACCCTGTC CTTGAACATGGCCATCTTTGCTTCTGTTTGCCTGGCCTCACGCCTCCCCCGGTCACTCCA CGCCTTCATTATGGTGACGTTTGCTATCCAGATCTTTGCACTGTGGCCCATGTTACAGAA GAAACTGAAGGCGTACACCCCGCGAAGCTATGTAGGGGTCACTTTGCTTTTTGCCTTTTC TGCCTTTGGAGGTCTTCTGTCCATTAGTGCTGTGGGAGCCATACTCTTTGCTCTCCTGCT GTTTTCCATCTCCTGTCTCTGCCCTTACTACCTCATTCATCTGCAGCTTTTTAAGGAAAA TATTCACGGGCCTTGGGATGAAGCTGAAATCAAGGAAGACTTGTCTAGGTTCCTTAGCTA ATCTAGGGACCTTCATTTCATTATTAAGACCAGCGGAAAGACTTCGAGCCTAACCGGTGC AACATTTAAAGGCAGGGGTCCACTCTTGAAGCAGCCGACTGATCTAGATGGTAGAGCTGA GATCAGAAGAAAGCATTTAACCAAGGGCTTAGGGGAGGGTGCTTGTCCTTCAGTTATTTT GTGACTGAAAAAGCTTTCTGGTGCTGTTATGAATTATTGTCCTTTATTCCCTGTAACAAA TGATGTAATGTGGGTTCTGCTTATTAAAAAAGTAATATCCAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008179 unedited CGCGTTCNGATTTGTATACGACTCACTATAGGCGGACCGCGATTCAGATCTGGTACCGGT CCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGTCTCGGTCGTGCACCCCCCTAAAG AGCTTGGTGCAGGAATTTAGCTGTGTTGTCCTGACCCTCAGGACAAACTCATCTAATGCA GGCTCAGCTTGTACTGAAGGCTTTGTAAACAAGGAAAGTTCTGTAGGGTATCAGCATGGC ATTTCCAGAGAGTTCTTAATCTACAGATTTATCAAGAACCAGAACCCCATGGACGTCCCT GAGGGAGATTGAGCCAACCTGGATTTGAGGAAAGAATGAGATAATGTGTGCTCAACGTGT AACAGACACCCCAGAAGTCAAGTGGCAGAAGGTTTTATATGAGCGGCAGCCGTTCCCTGA TAACTATGTTGACCAGAGGTTCCTGGAAGAACTCCGGAAAAACATCTATGCCCGGAAGTA CCAGTACTGGGCTGTGGTATTTGAGTCCAGTGTGGTGATACAGCAGCTGTGCAGTGTCTG TGTTTTTGTAGTGATCTGGTGGTACATGGATGAGGGTCTTCTGGCTCCCCAGTGGCTTTT TGGGACCGGACTGGCATCTTCCTTGGTTGGGTATGTTTTATTCGATCTTATCGATGGAGG TGATGGACNGAAGAAGAGTGGGCGGACCCGGTGGGCCGACCTGAAGAGTACTCTGGTCTT TATCACTTTCACTTACGGTTTTTCACCTGTGCTAAAGACCCTGACGGAGTCTGTCAGTAC AGACACTATCTACGCCATGGCAGTCTTTATGCCCTGTAGGCCATCTCATCTTCTTTTGAT ACCGGTGCCCATGCTGCTATTGTATCCAGCACCCTGTCTTGGACATGAGCATCCTTGCTT CTGTTTGCCC
Restriction Sites	Please inquire
ACCN	BC008179
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC008179.1, AAH08179.1
RefSeq Size	1435 bp
Locus ID	5279
Protein Families	Transmembrane
Protein Pathways	Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
Gene Summary	This gene encodes an endoplasmic reticulum associated protein that is involved in glycosylphosphatidylinositol (GPI) lipid anchor biosynthesis. The GPI lipid anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. The encoded protein is one subunit of the GPI N-acetylglucosaminyl (GlcNAc) transferase that transfers GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. Two alternatively spliced transcripts that encode the same protein have been found for this gene. A pseudogene on chromosome 11 has also been characterized. [provided by RefSeq, Jul 2008]

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