GPAA1 (BC003171) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	anchor attachment protein 1 (Gaa1p, yeast) homolog; GAA1; glycophosphatidylinositol anchor attachment 1; glycosylphospha; glycosylphosphatidylinositol anchor attachment protein 1; GPAA1P anchor attachment protein 1 homolog; GPI transamidase subunit; hGAA1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC003171 edited CCCGGGTCTGACAGGAGCAGCCTGTGGGCACCGCGGCGGTAGTTGGAGGCGGGAGAGGGT CCGTAGCCGCGCCGCCCTGCCCCGCCATGGGCCTCCTGTCGGACCCGGTTCGCCGGCGCG CGCTCGCCCGCCTAGTGCTGCGCCTCAACGCGCCGTTGTGCGTGCTGAGCTACGTGGCGG GCATCGCCTGGTTCTTGGCGCTGGTTTTCCCGCCGCTGACCCAGCGCACTTACATGTCGG AGAACGCCATGGGCTCCACCATGGTGGAGGAGCAGTTTGCGGGCGGAGACCGTGCCCGGG CTTTTGCCCGGGACTTCGCCGCCCACCGCAAGAAGTCGGGGGCTCTGCCAGTGGCCTGGC TTGAACGGACGATGCGGTCAGTAGGGCTGGAGGTCTACACGCAGAGTTTCTCCCGGAAAC TGCCCTTCCCAGATGAGACCCACGAGCGCTATATGGTGTCGGGCACCAACGTGTACGGCA TCCTGCGGGCCCCGCGTGCTGCCAGCACCGAGTCGCTTGTGCTCACCGTGCCCTGTGGCT CTGACTCTACCAACAGCCAGGCTGTGGGGCTGCTGCTGGCACTGGCTGCCCACTTCCGGG GGCAGATTTATTGGGCCAAAGATATCGTCTTCCTGGTAACAGAACATGACCTTCTGGGCA CTGAGGCTTGGCTTGAAGCCTACCACGATGTCAATGTCACTGGCATGCAGTCGTCTCCCC TGCAGGGCCGAGCTGGGGCCATTCAGGCAGCCGTGGCCCTGGAGCTGAGCAGTGATGTGG TCACCAGCCTCGATGTGGCCGTGGAGGGGCTTAACGGGCAGCTGCCCAACCTTGACCTGC TCAATCTCTTCCAGACCTTCTGCCAGAAAGGGGGCCTGTTGTGCACGCTTCAGGGCAAGC TGCAGCCCGAGGACTGGACATCATTGGATGGACCGCTGCAGGGCCTGCAGACACTGCTGC TCATGGTTCTGCGGCAGGCCTCCGGCCGCCCCCACGGCTCCCATGGCCTCTTCCTGCGCT ACCGTGTGGAGGCCCTAACCCTGCGTGGCATCAATAGCTTCCGCCAGTACAAGTATGACC TGGTGGCAGTGGGCAAGGCTTTGGAGGGCATGTTCCGCAAGCTCAACCACCTCCTGGAGC GCCTGCACCAGTCCTTCTTCCTCTACTTGCTCCCCGGCCTCTCCCGCTTCGTCTCCATCG GCCTCTACATGCCCGCTGTCGGCTTCTTGCTCCTGGTCCTTGGTCTCAAGGCTCTGGAAC TGTGGATGCAGCTGCATGAGGCTGGAATGGGCCTTGAGGAGCCCGGGGGTGCCCCTGGCC CCAGTGTACCCCTTCCCCCATCACAGGGTGTGGGGCTGGCCTCGCTCGTGGCACCTCTGC TGATCTCACAGGCCATGGGACTGGCCCTCTATGTCCTGCCAGTGCTGGGCCAACACGTTG CCACCCAGCACTTCCCAGTGGCAGAGGCTGAGGCTGTGGTGCTGACACTGCTGGCGATTT ATGCAGCTGGCCTGGCCCTGCCCCACAATACCCACCGGGTGGTAAGCACACAGGCCCCAG ACAGGGGCTGGATGGCACTGAAGCTGGTAGCCCTGATCTACCTAGCACTGCAGCTGGGCT GCATCGCCCTCACCAACTTCTCACTGGGCTTCCTGCTGGCCACCACCATGGTGCCCACTG CTGCGCTTGCCAAGCCTCATGGGCCCCGGACCCTCTATGCTGCCCTGCTGGTGCTGACCA GCCCGGCAGCCACGCTCCTTGGCAGCCTGTTCCTGTGGCGGGAGCTGCAGGAGGCGCCAC TGTCACTGGCCGAGGGCTGGCAGCTCTTCCTGGCAGCGCTAGCCCAGGGTGTGCTGGAGC ACCACACCTACGGCGCCCTGCTCTTCCCACTGCTGTCCCTGGGCCTCTACCCCTGCTGGC TGCTTTTCTGGAATGTGCTCTTCTGGAAGTGAGATCTGCCTGTCCGGGCTGGGACAGAGA CTCCCCAAGGACCCCATTCTGCCTCCTTCTGGGGAAATAAATGAGTGTCTGTTTCAGCAG CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC003171 unedited CGCGGGTCAAATTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCCGG GTCTGACAGGAGCAGCCTGTGGGCACCGCGGCGGTAGTTGGAGGCGGGAGAGGGTCCGTA GCCGCGCCGCCCTGCCCCGCCATGGGCCTCCTGTCGGACCCGGTTCGCCGGCGCGCGCTC GCCCGCCTAGTGCTGCGCCTCAACGCGCCGTTGTGCGTGCTGAGCTACGTGGCGGGCATC GCCTGGTTCTTGGCGCTGGTTTTCCCGCCGCTGACCCAGCGCACTTACATGTCGGAGAAC GCCATGGGCTCCACCATGGTGGAGGAGCAGTTTGCGGGCGGAGACCGTGCCCGGGCTTTT GCCCGGGACTTCGCCGCCCACCGCAAGAAGTCGGGGGCTCTGCCAGTGGCCTGGCTTGAA CGGACGATGCGGTCAGTAGGGCTGGAGGTCTACACGCAGAGTTTCTCCCGGAAACTGCCC TTCCCAGATGAGACCCACGAGCGCTATATGGTGTCGGGCACCAACGTGTACGGCATCCTG CGGGCCCCGCGTGCTGCCAGCACCGAGTCGCTTGTGCTCACCGTGCCCTGTGGCTCTGAC TCTACCAACAGCCAGGCTGTGGGGCTGCTGCTGGCACTGGCTGCCCACTTCCGGGGGCAG ATTTATTGGGCCAAAGATATCGTCTTCCTGGTAACAGAACATGACCTTCTGGGCACTGAG GCTTGGCTTGAAGCCTACCACGATGTCAATGTCACTGGGCAGGCAGTTCGTCTTCCCCTG CAGGGGCCGAGCCTGGGGCCATATCAGGCACACGTTGCCCCTGGTAGCTGTAACAATGGT GTGGTTACCCAACCCTCCATGTGGCCGTGGNAGGGGCCTTAACCGGGCAGCTTGCCCAAC TTTGAACTGT
Restriction Sites	Please inquire
ACCN	BC003171
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC003171.1, AAH03171.1
RefSeq Size	2081 bp
RefSeq ORF	1863 bp
Locus ID	8733
Protein Families	Transmembrane
Protein Pathways	Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
Gene Summary	Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.