ALG10 (BC070347) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	alpha2-glucosyltransferase; asparagine-linked glycosylation 10 homolog (yeast, alpha-1,2-glucosyltransferase); asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog (S. p; derepression of ITR1 expression 2 homolog; DIE2; FLJ14751; KCR1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC070347 edited CCGCCTAGCGCGCCCATTTCGAGCCCAAGTTTCCAGCTCGGGTTTCCAGGCTCAGAATTT TCCAGGAGTAGGTCCTTGGGCAGTGGCTGTGGGAGCTGGAATGGCGCAGCTGGAAGGTTA CTATTTCTCGGCCGCCTTGAGCTGTACCTTTTTAGTATCCTGCCTCCTCTTCTCCGCCTT CAGCCGGGCGTTGCGAGAGCCCTACATGGACGAGATCTTCCACCTGCCTCAGGCGCAGCG CTACTGTGAGGGCCATTTCTCCCTTTCCCAGTGGGATCCCATGATTACTACATTACCTGG CTTGTACCTGGTGTCAATTGGAGTGATCAAACCTGCCATTTGGATCTTTGGATGGTCTGA ACATGTTGTCTGCTCCATTGGGATGCTCAGATTTGTTAATCTTCTCTTCAGTGTTGGCAA CTTCTATTTACTATATTTGCTTTTCTGCAAGGTACAACCCAGAAACAAGGCTGCCTCAAG TATCCAGAGAGTCTTGTCAACATTAACACTAGCAGTATTTCCAACACTTTATTTTTTTAA CTTCCTTTATTATACAGAAGCAGGATCTATGTTTTTTACTCTTTTTGCGTATTTGATGTG TCTTTATGGAAATCATAAAACTTCAGCCTTCCTTGGATTTTGTGGCTTCATGTTTCGGCA AACAAATATCATCTGGGCTGTCTTCTGTGCAGGAAATGTCATTGCACAAAAGTTAACGGA GGCTTGGAAAACTGAGCTACAAAAGAAGGAAGACAGACTTCCACCTATTAAAGGACCATT TGCAGAATTCAGAAAAATTCTTCAGTTTCTTTTGGCTTATTCCATGTCCTTTAAAAACTT GAGTATGCTTTTGCTTCTGACTTGGCCCTACATCCTTCTGGGATTTCTGTTTTGTGCTTT TGTAGTAGTTAATGGTGGAATTGTTATTGGCGATCGGAGTAGTCATGAAGCCTGTCTTCA TTTTCCTCAACTATTCTACTTTTTTTCATTTACTCTCTTTTTTTCCTTTCCTCATCTCCT GTCTCCTAGCAAAATTAAGACTTTTCTTTCCTTAGTTTGGAAACGTAGAATTCTGTTTTT TGTGGTTACCTTAGTCTCTGTGTTTTTAGTTTGGAAATTCACTTATGCTCATAAATACTT GCTAGCAGACAATAGACATTATACTTTCTATGTGTGGAAAAGAGTTTTTCAAAGATATGA AACTGTAAAATATTTGTTAGTTCCAGCCTATATATTTGCTGGTTGGAGTATAGCTGACTC ATTGAAATCAAAGTCAATTTTTTGGAATTTAATGTTTTTCATATGCTTGTTCACTGTTAT AGTTCCTCAGAAACTGCTGGAATTTCGTTACTTCATTTTACCTTATGTCATTTATAGGCT TAACATACCTCTGCCTCCCACATCCAGACTCATTTGTGAACTGAGCTGCTATGCAGTTGT TAATTTCATAACTTTTTTCATCTTTCTGAACAAGACTTTTCAGTGGCCAAATAGTCAGGA CATTCAAAGGTTTATGTGGTAATATCAGTGATATTTCGAACTGTGAAAATGGACTTAATA ATTAGACCATTTCTACAAAGAACAACTGAATAGGTGGAAAACATGGAATTTCTTTTAGGT GCAGTGGTGGTCTTCAAATTACATTAGTTTTTTTTATATATATTTTAAACATATGTAAGA AATTAAGTGGCAAAGAACTGAGAAAGCTTAAGACCTGCTTCAAAAGCCTGAAAAATGGAA AAATAAAATTGTTTTCAGATATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC070347 unedited NGGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCAATCTGGTACCGGTCC GGAATTCCCGGGATCCGCCTAGCGCGCCCATTTCGAGCCCAACTTTCCAGCTCGGGTTTC CAGGCTCAGAATTTTCCAGGAGTAGGTCCTTGGGCAGTGGCTGTGGGAGCTGGAATGGCG CAGCTGGAAGGTTACTATTTCTCGGCCGCCTTGAGCTGTACCTTTTTAGTATCCTGCCTC CTCTTCTCCGCCTTCAGCCGGGCGTTGCGAGAGCCCTACATGGACGAGATCTTCCACCTG CCTCAGGCGCAGCGCTACTGTGAGGGCCATTTCTCCCTTTCCCAGTGGGATCCCATGATT ACTACATTACCTGGCTTGTACCTGGTGTCAATTGGAGTGATCAAACCTGCCATTTGGATC TTTGGATGGTCTGAACATGTTGTCTGCTCCATTGGGATGCTCAGATTTGTTAATCTTCTC TTCAGTGTTGGCAACTTCTATTTACTATATTTGCTTTTCTGCAAGGTACAACCCAGAAAC AAGGCTGCCTCAAGTATCCAGAGAGTCTTGTCAACATTAACACTAGCAGTATTTCCAACA CTTTATTTTTTTAACTTCCTTTATTATACAGAAGCAGGATCTATGTTTTTTACTCTTTGT GCGTATTTGATGTGTCTTTATGGGAATCATANGACTTCAGCCTTCCTTGGATTATGTGGC TTCATGTTTCGGCAAACAAATATCATCTGGGCTGTCTTCTGTGCANGANATGTCATTGCT CNACAGTTAACGGGAGCTTGGAAAACTGAGCTACAAAGAAGGAGACAGACTTCACCTATT ATAGACCATTGCAGAGTCAGAAAATTCGTCAGTTTCGTTTGGCCTATCCATGTCCTTTAA AAACTGATATGCTTTTGCTT
Restriction Sites	Please inquire
ACCN	BC070347
Insert Size	1820 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC070347.1, AAH70347.1
RefSeq Size	1794 bp
RefSeq ORF	1419 bp
Locus ID	84920
Protein Families	Transmembrane
Protein Pathways	Metabolic pathways, N-Glycan biosynthesis
Gene Summary	This gene encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel. [provided by RefSeq, Jan 2010]

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