DAZL (BC027595) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DAZH; DAZL1; DAZLA; deleted in azoospermia-like; deleted in azoospermia-like autosomal; germline specific RNA binding protein; MGC26406; spermatogenesis gene on the Y-like autosomal; SPGYLA
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC027595 edited ATTGGCTGAGGCCGATACCACGCGCCCCGATACCCGGCGCAGGAGCCACCTCCCTGAGCC CCGCGGACCACGCCTCAGTCCGCCTGCGCTCCTCAGCCTGACGGTCCGCCTTTCGGGGCT CCTCAGCCTTGTCACCCGCTCTTGGTTTTCCTTTTCTCTTCATCTTTGGCTCCTTTGACC ACTCGAAGCCGCGCAGCGGGTTCCAGTGGACCTCACAGCAGCCCCAGAAGTGGTGCGCCA AGCACAGCCTCTGCTCCTCCTCGAGCCGGTCGGGAACTGCTGCCTGCCGCCATCATGTCT ACTGCAAATCCTGAAACTCCAAACTCAACCATCTCCAGAGAGGCCAGCACCCAGTCCTCA TCAGCTGCAACCAGCCAAGGCTATATTTTACCAGAAGGCAAAATCATGCCAAACACTGTT TTTGTTGGAGGAATTGATGTTAGGATGGATGAAACTGAGATTAGAAGCTTCTTTGCTAGA TATGGTTCAGTGAAAGAAGTGAAGATAATCACTGATCGAACTGGTGTGTCCAAAGGCTAT GGATTTGTTTCATTTTTTAATGACGTGGATGTGCAGAAGATAGTAGAATCACAGATAAAT TTCCATGGTAAAAAGCTGAAGCTGGGCCCTGCAATCAGGAAACAAAATTTATGTGCTTAT CATGTGCAGCCACGTCCTTTGGTTTTTAATCATCCTCCTCCACCACAGTTTCAGAATGTC TGGACTAATCCAAACACTGAAACTTATATGCAGCCCACAACCACGATGAATCCTATAACT CAGTATGTTCAGGCATATCCTACTTACCCAAATTCACCAGTTCAGGTCATCACTGGATAT CAGTTGCCTGTATATAATTATCAGATGCCACCACAGTGGCCTGTTGGGGAGCAAAGGAGC TATGTTGTACCTCCGGCTTATTCAGCTGTTAACTACCACTGTAATGAAGTTGATCCAGGA GCTGAAGTTGTGCCAAATGAATGTTCAGTTCATGAAGCTACTCCACCCTCTGGAAATGGC CCACAAAAGAAATCTGTGGACCGAAGCATACAAACGGTGGTATCTTGTCTGTTTAATCCA GAGAACAGACTGAGAAACTCTGTTGTTACTCAAGATGACTACTTCAAGGATAAAAGAGTG CATCACTTTAGAAGAAGTCGGGCAATGCTTAAATCTGTTTGATCCTCCTGGCTTATCTAG TTACATGGGAAGTTGCTGGTTTTGAATATTAAGCTAAAAGGTTTCCACTATTATAGAAAT TCTGAATTTTGGTAAATCACACTCAAACTTTGTGTATAAGTTGTATTATTAGACTCTCTA GTTTTATCTTAAACTGTTCTTCATTAGATGTTTATTTAGAAACTGGTTCTGTGTTGAAAT ATAGTTGAAAGTAAAAAAATAATTGAGACTGAAAGAAACTAAGATTTATCTGCAAGGATT TTTTAAAAATTGGCATTTTAAGTGTTTAAAAGCAAATACTGATTTTCAAAAAAATGTTTT TAAAAACCTATTTTGAAAGGTCAGAATTTTGTTGGTCTGAATACAAACATTTCACTTCTC CAACAAGTACCTGTGAACAGTACAGTATTTACAGTATTGAGCTTTGCATTTATGATTTCT CCAGAAATTTACCACAAAAGCAAAATTTTTAAAACTGCATTTTTAATCAGTGGAACTCAA TATATAGTTAGCTTTATTGAAGTCTTCTTATCTAAACCCAGCAAAACAGATTCAAAGCAA ACAGTCCAATCAGTGGGTCATATGTTTATTCAAAATATTTTATCTTTTAGCTAGAATCCA CACATATATATCCTATTTGATTAGGGTAGTAATTAGGATAACTAAAATTCTGGGCCTAAT TTTTTAAAGAATCCAAGACAAACTAAACTTTACTAGGTACATAAGCTTCTCAATGAGTCA CCATTCTTCTTTTTTGTAAAAACTTTTTTCTTTGAAATGCTAAACTTGGCTGTATGTCAA ATTGTGCAAAATATTGGTATTAAAGAATGCTGCAACTTTAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC027595 unedited GTTTGGGATTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATACAT TATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGATTGG CTGAGGCCGATACCACGCGCCCCGATACCCGGCGCATGAGCCACCTCCCTGAGCCCCGCG GACCACGCCTCAGTCCGCCTGCGCTCCTCAGCCTGACGGTCCGCCTTTCGGGGCTCCTCA GCCTTGTCACCCGCTCTTGGTTTTCCTTTTCTCTTCATCTTTGGCTCCTTTGACCACTCG AAGCCGCGCAGCGGGTTCCAGTGGACCTCACAGCAGCCCCAGAAGTGGTGCGCCAAGCAC AGCCTCTGCTCCTCCTCGAGCCGGTCGGGAACTGCTGCCTGCCGCCATCATGTCTACTGC AAATCCTGANACTCCAAACTCAACCATCTCCAGAGAGGCCAGCACCCAGTCCTCATCAGC TGCAACCAGCCAAGGCTATATTTTACCAGAAGGCATAATCATGCCAAACACTGTTTTTGT TGGAGGAATTGATGTTAGGATGGATGAAACTGAGATTAGAAGCTTCTTTGCTAGATATGG TTCAGTGAAAGAAGTGAAGATAATCACTGATCGAACTGGTGTGTCCAAAGGCTATGGATT TGTTTCATTTTTTAATGACGTGGATGTGCAGAAGATAGTAGAATCACAGATAAATTTCCA TGGTAAAAAGCTGAAGCTGGGCCCCTGCATCAGGANACANAATTTATGTGCTTATCATGT GCAGCCACGTCCTTTGGNTTTTAATCATCCTCCTNCACCACAGTTTNCAGATGTCTGGAC TAATCCAACACTGAANCTTATATGCAGCCCCACACCACGATGAATCCTATNACTCAGTAT GTTCAGGCATATCCTACTTTACCAATTNCACAGTTCAGG
Restriction Sites	Please inquire
ACCN	BC027595
Insert Size	2038 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC027595.2, AAH27595.1
RefSeq Size	2033 bp
Locus ID	1618
Gene Summary	The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

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