PLOD1 (NM_000302) Human Untagged Clone

Name: PLOD1 (NM_000302) Human Untagged Clone
SKU: SC119956
Price: 5336.00

CAT＃: SC119956

PLOD1 (untagged)-Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1)

Datasheet

SDS

"NM_000302" in other vectors (6)

CNY 2,950.00

货期*

现货

规格

Cited in 1 publication.

Product images

经常一起买 (4)

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA362064

PLOD1 Antibody - C-terminal region

CNY 4,628.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EDS6; EDSKCL1; LH; LH1; LLH; PLOD
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for NM_000302 edited ATGCGGCCCCTGCTGCTACTGGCCCTGCTGGGCTGGCTGCTGCTGGCCGAAGCGAAGGGC GACGCCAAGCCGGAGGACAACCTTTTAGTCCTCACGGTGGCCACTAAGGAGACCGAGGGA TTCCGTCGCTTCAAGCGCTCAGCTCAGTTCTTCAACTACAAGATCCAGGCGCTTGGCCTA GGGGAGGACTGGAATGTGGAGAAGGGGACGTCGGCAGGTGGAGGGCAGAAGGTCCGGCTG CTGAAGAAAGCTCTGGAGAAGCACGCAGACAAGGAGGATCTGGTCATTCTCTTCGCAGAC AGCTATGACGTGCTGTTTGCATCGGGGCCCCGGGAGCTCCTGAAGAAGTTCCGGCAGGCC AGGAGCCAGGTGGTCTTCTCTGCTGAGGAGCTCATCTACCCAGACCGCAGGCTGGAGACC AAGTATCCGGTGGTGTCCGATGGCAAGAGGTTCCTGGGCTCTGGAGGCTTCATCGGTTAT GCCCCCAACCTCAGCAAACTGGTGGCCGAGTGGGAGGGCCAGGACAGCGACAGCGATCAG CTGTTTTACACCAAGATCTTCTTGGACCCGGAGAAGAGGGAGCAGATCAATATCACCCTG GACCACCGCTGCCGTATCTTCCAGAACCTGGATGGAGCCTTGGATGAGGTCGTGCTCAAG TTTGAAATGGGCCATGTGAGAGCGAGGAACCTGGCCTATGACACCCTCCCGGTCCTGATC CATGGCAACGGGCCAACCAAGCTGCAGTTGAACTACCTGGGCAACTACATCCCGCGCTTC TGGACCTTCGAAACAGGCTGCACCGTGTGTGACGAAGGCTTGCGCAGCCTCAAGGGCATT GGGGATGAAGCTCTGCCCACGGTCCTGGTCGGCGTGTTCATCGAACAGCCCACGCCGTTT GTGTCCCTGTTCTTCCAGCGGCTCCTGCGGCTCCACTACCCCCAGAAACACATGCGACTT TTCATCCACAACCACGAGCAGCACCACAAGGCTCAGGTGGAAGAGTTCCTGGCACAGCAT GGCAGCGAGTACCAGTCTGTGAAGCTGGTGGGCCCTGAGGTGCGGATGGCGAATGCAGAT GCCAGGAACATGGGCGCAGACCTGTGCCGGCAGGACCGCAGCTGCACCTACTACTTCAGC GTGGATGCTGACGTGGCCCTGACCGAGCCCAACAGCCTGCGGCTGCTGATCCAACAGAAC AAGAACGTCATTGCCCCGCTGATGACCCGGCATGGGAGGCTGTGGTCGAACTTCTGGGGG GCTCTCAGTGCAGATGGCTACTATGCCCGTTCCGAGGACTACGTGGACATTGTGCAGGGG CGGCGTGTTGGTGTCTGGAATGTGCCCTATATTTCAAACATCTACTTGATCAAGGGCAGT GCCCTGCGGGGTGAGCTGCAGTCCTCAGATCTCTTCCACCACAGCAAGCTGGACCCCGAC ATGGCCTTCTGTGCCAACATCCGGCAGCAGGATGTGTTCATGTTCCTGACCAACCGGCAC ACCCTTGGCCATCTGCTCTCCCTAGACAGCTACCGCACCACCCACCTGCACAACGACCTC TGGGAGGTGTTCAGCAACCCCGAGGACTGGAAGGAGAAGTACATCCACCAGAACTACACC AAAGCCCTGGCAGGGAAGCTGGTGGAGACGCCCTGCCCGGATGTCTATTGGTTCCCCATC TTCACGGAGGTGGCCTGTGATGAGCTGGTGGAGGAGATGGAGCACTTTGGCCAGTGGTCT CTGGGCAACAACAAGGACAACCGCATCCAGGGTGGCTACGAGAACGTGCCGACTATTGAC ATCCACATGAACCAGATCGGCTTTGAGCGGGAGTGGCACAAATTCCTGCTGGAGTACATT GCGCCCATGACGGAGAAGCTCTACCCCGGCTACTACACCAGGGCCCAGTTTGACCTGGCC TTTGTCGTCCGCTACAAGCCTGATGAGCAGCCCTCACTGATGCCACACCATGATGCCTCC ACCTTCACCATCAACATCGCCCTGAACCGAGTCGGGGTGGATTACGAGGGCGGGGGCTGT CGGTTCCTGCGCTACAACTGTTCCATCCGAGCCCCAAGGAAGGGCTGGACCCTCATGCAC CCTGGACGACTCACGCATTACCATGAGGGGCTCCCCACCACCAGGGGCACCCGCTACATC GCAGTCTCCTTCGTCGATCCCTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_000302 unedited NGGTGTTCAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGGAC CTGCGGCCCCGNTCGCGAGTTTCCAGCCCTGCGAGCGCCGCCGGGTCGGCCGATCGTCCC CCATACCTCGGCCATGCGGCCCCTGCTGCTACTGGCCCTGCTGGGCTGGCTGCTGCTGGC CGAAGCGAAGGGCGACGCCAAGCCGGAGGACAACCTTTTAGTCCTCACGGTGGCCACTAA GGAGACCGAGGGATTCCGTCGCTTCAAGCGCTCAGCTCAGTTCTTCAACTACAAGATCCA GGCGCTTGGCCTAGGGGAGGACTGGAATGTGGAGAAGGGGACGTCGGCAGGTGGAGGGCA GAAGGTCCGGCTGCTGAAGAAAGCTCTGGAGAAGCACGCAGACAAGGAGGATCTGGTCAT TCTCTTCGCAGACAGCTATGACGTGCTGTTTGCATCGGGGCCCCGGGAGCTCCTGAAGAA GTTCCGGCAGGCCAGGAGCCAGGTGGTCTTCTCTGCTGAGGAGCTCATCTACCCAGACCG CAGGCTGGAGACCAAGTATCCGGTGGTGTCCGATGGCAAGAGGTTCCTGGGCTCTGGAGG CTTCATCGGTTATGCCCCCAACCTCAGCAAACTGGTGGCCGAGTGGGAGGGCCAGGACAG CGACAGCGATCAGCTGTTTTACACCAAGATCTTCTTGGACCCGGAGAAGAAGGAGCAGAT CAATATCACCCTGGACCACCGCTGCCGTATCTTCCAGACCTGGATGGAGCCTTGGATGAA GTCGTGCTCAAGTTTGAAATGGCCATGTGAGAGCGAAGAACCTGGCCTATGACACCCTCC CCGTCCTGATCCATGGCAAACGGGCCACCAAGCTGCAGTTGAACTACCTGGNGCACTACA TNCCG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_000302 unedited CTTTATAGAACTCTCCGTTTCTGACGGTCTGTCCCTCTCTTCANAGCATCCAGGACAGGG TGACCCAGTGTGGTGTGTGGACCTGTCACTCCCCTCCCCACAGGCAAGTAGAAGATCGAG CTGTGCACAGATGCCTCTTCTGTCTCCCAAGAGGTTCTCTGGAGTCGCTGTCTCCAGGGG ACTACGGTTTGCTCTCATGACCAGCCATGGAGGCAGAAGTCCCAGACTCAGGGCAGCCCT TGGAGTCTGATCCAGGTGTCTTTACCCCAAAACTTGAAGCAGAAGTCCCTGGTGCAACAC CTGTGCCTGGGAGAAGCTCTGGCCATGGCTTCTCACCCAGACAGTCTTTCTGGGCAACTT GGGGAAGCCCCTGTTCTGCTCAAGTCTCACCCCATGGAAGAGGTGGGGGAAGGGGGCCTT GGTTTTTCAGGAAGACAGGTTGGAGAGCACGAGTCACTACAAAGCAGTAAAAGTGAATGG TGTCTCCAGGGGCTGGGTCCAGAACACCACGGAGAGCCCCAGCCATAAAGGTGTGTTCCG CCTCTGGCCTGCAGGAATCTCTTTGAATCTCTNTGATTGGTGGCTCCAAGAGCAATGGGA AGTCAACAGCCCAGAGGCTGGACTGNGTTCCCTGGGGACCCCGAGTCCCANNAGCTGCTG GGCAGTGGTTGTCGGCAAAGAAAGAAGGTCCCAGAGGGTCANGCCTGCCCAATANGGATC GACGAAGGAGACTGCGATGTACCGGGTGCCCCTGTTGGTGGGGAGCCCCTCATGGTATGC GTGAGTCGTCCAGGGTGCATGAGGGTTCACCCTTNCCTTGGGGCTCGAATGGACCAGTTG TACGCANGACCCGACAGNCCCCGCCTCGTNATCCACCCGACTGCGTCAGGGCCATTGTGA TGGTGAAGGCTGAGGCCTCATGGTGTGGCACTCATGAAGCCTGCTATCAAGTCTGATACG AACACAAGGCAGGTCAACTGGGCCCTGTGTATTACCGGGTAAAGCTTCCCTCATGGCCAT
Restriction Sites	NotI-NotI
ACCN	NM_000302
Insert Size	3180 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000302.2, NP_000293.2
RefSeq Size	3004 bp
RefSeq ORF	2184 bp
Locus ID	5351
UniProt ID	Q02809
Domains	2OG-FeII_Oxy, P4Hc
Protein Families	Druggable Genome
Protein Pathways	Lysine degradation
Gene Summary	Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Aberrantly expressed PLOD1 promotes cancer aggressiveness in bladder cancer: a potential prognostic marker and therapeutic target ,Yamada, Y;Kato, M;Arai, T;Sanada, H;Uchida, A;Misono, S;Sakamoto, S;Komiya, A;Ichikawa, T;Seki, N;, Mol Oncol ,PubMed ID 31199049 [PLOD1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC201969	PLOD1 (Myc-DDK-tagged)-Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,320.00 CNY 5,800.00
RC201969L1	Lenti ORF clone of Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
RC201969L2	Lenti ORF clone of Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
RC201969L3	Lenti ORF clone of Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
RC201969L4	Lenti ORF clone of Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
RG201969	PLOD1 (tGFP-tagged) - Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,370.00

其它PLOD1产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies