KEAP1 (NM_012289) Human Untagged Clone

Name: KEAP1 (NM_012289) Human Untagged Clone
SKU: SC111946
Price: 4656.00

CAT＃: SC111946

KEAP1 (untagged)-Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2

Datasheet

SDS

"NM_012289" in other vectors (6)

CNY 2,950.00

货期*

现货

规格

Cited in 3 publications.

Product images

经常一起买 (4)

TA502059

KEAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	INrf2; KLHL19
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_012289, the custom clone sequence may differ by one or more nucleotides ATGCAGCCAGATCCCAGGCCTAGCGGGGCTGGGGCCTGCTGCCGATTCCTGCCCCTGCAGTCACAGTGCC CTGAGGGGGCAGGGGACGCGGTGATGTACGCCTCCACTGAGTGCAAGGCGGAGGTGACGCCCTCCCAGCA TGGCAACCGCACCTTCAGCTACACCCTGGAGGATCATACCAAGCAGGCCTTTGGCATCATGAACGAGCTG CGGCTCAGCCAGCAGCTGTGTGACGTCACACTGCAGGTCAAGTACCAGGATGCACCGGCCGCCCAGTTCA TGGCCCACAAGGTGGTGCTGGCCTCATCCAGCCCTGTCTTCAAGGCCATGTTCACCAACGGGCTGCGGGA GCAGGGCATGGAGGTGGTGTCCATTGAGGGTATCCACCCCAAGGTCATGGAGCGCCTCATTGAATTCGCC TACACGGCCTCCATCTCCATGGGCGAGAAGTGTGTCCTCCACGTCATGAACGGTGCTGTCATGTACCAGA TCGACAGCGTTGTCCGTGCCTGCAGTGACTTCCTGGTGCAGCAGCTGGACCCCAGCAATGCCATCGGCAT CGCCAACTTCGCTGAGCAGATTGGCTGTGTGGAGTTGCACCAGCGTGCCCGGGAGTACATCTACATGCAT TTTGGGGAGGTGGCCAAGCAAGAGGAGTTCTTCAACCTGTCCCACTGCCAACTGGTGACCCTCATCAGCC GGGACGACCTGAACGTGCGCTGCGAGTCCGAGGTCTTCCACGCCTGCATCAACTGGGTCAAGTACGACTG CGAACAGCGACGGTTCTACGTCCAGGCGCTGCTGCGGGCCGTGCGCTGCCACTCGTTGACGCCGAACTTC CTGCAGATGCAGCTGCAGAAGTGCGAGATCCTGCAGTCCGACTCCCGCTGCAAGGACTACCTGGTCAAGA TCTTCGAGGAGCTCACCCTGCACAAGCCCACGCAGGTGATGCCCTGCCGGGCGCCCAAGGTGGGCCGCCT GATCTACACCGCGGGCGGCTACTTCCGACAGTCGCTCAGCTACCTGGAGGCTTACAACCCCAGTGACGGC ACCTGGCTCCGGTTGGCGGACCTGCAGGTGCCGCGGAGCGGCCTGGCCGGCTGCGTGGTGGGCGGGCTGT TGTACGCCGTGGGCGGCAGGAACAACTCGCCCGACGGCAACACCGACTCCAGCGCCCTGGACTGTTACAA CCCCATGACCAATCAGTGGTCGCCCTGCGCCCCCATGAGCGTGCCCCGTAACCGCATCGGGGTGGGGGTC ATCGATGGCCACATCTATGCCGTCGGCGGCTCCCACGGCTGCATCCACCACAACAGTGTGGAGAGGTATG AGCCAGAGCGGGATGAGTGGCACTTGGTGGCCCCAATGCTGACACGAAGGATCGGGGTGGGCGTGGCTGT CCTCAATCGTCTCCTTTATGCCGTGGGGGGCTTTGACGGGACAAACCGCCTTAATTCAGCTGAGTGTTAC TACCCAGAGAGGAACGAGTGGCGAATGATCACAGCAATGAACACCATCCGAAGCGGGGCAGGCGTCTGCG TCCTGCACAACTGTATCTATGCTGCTGGGGGCTATGATGGTCAGGACCAGCTGAACAGCGTGGAGCGCTA CGATGTGGAAACAGAGACGTGGACTTTCGTAGCCCCCATGAAGCACCGGCGAAGTGCCCTGGGGATCACT GTCCACCAGGGGAGAATCTACGTCCTTGGAGGCTATGATGGTCACACGTTCCTGGACAGTGTGGAGTGTT ACGACCCAGATACAGACACCTGGAGCGAGGTGACCCGAATGACATCGGGCCGGAGTGGGGTGGGCGTGGC TGTCACCATGGAGCCCTGCCGGAAGCAGATTGACCAGCAGAACTGTACCTGTTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_012289 unedited TCACTATATTTGTATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGAGCGC GGCCATGGCGGGGTCCCTGAGTGCCAGAGGTGGTGGTGTTGCTTATCTTCTGGAACCCCA TGCAGCCAGATCCCAGGCCTAGCGGGGCTGGGGCCTGCTGCCGATTCCTGCCCCTGCAGT CACAGTGCCCTGAGGGGGCAGGGGACGCGGTGATGTACGCCTCCACTGAGTGCAAGGCGG AGGTGACGCCCTCCCAGCATGGCAACCGCACCTTCAGCTACACCCTGGAGGATCATACCA AGCAGGCCTTTGGCATCATGAACGAGCTGCGGCTCAGCCAGCAGCTGTGTGACGTCACAC TGCAGGTCAAGTACCAGGATGCACCGGCCGCCCAGTTCATGGCCCACAAGGTGGTGCTGG CCTCATCCAGCCCTGTCTTCAAGGCCATGTTCACCAACGGGCTGCGGGAGCAGGGCATGG AGGTGGTGTCCATTGAGGGTATCCACCCCAAGGTCATGGAGCGCCTCATTGAATTCGCCT ACACGGCCTCCATCTCCATGGGCGAGAAGTGTGTCCTCCACGTCATGAACGGTGCTGTCA TGTACCAGATCGACAGCGTTGTCCGTGCCTGCAGTGACTTCCTGGTGCAGCAGCTGGACC CCAGCAATGCCATCGGCATCGCCCACTTCGCTGAGCAGATTGGCTGTGTGGAGTTGCACC ACCGTGCCCGGGGAGTACATCTACATGCATTTTGGGGAGGTTGGCCAACCAAGAGGAGTT CTTCACCCTGTCCCACTGGCCACTGGGTGACCCTCATCAGCCGGGACGACCTGNACGTGC GCTGCGAGTCCCAAGTCTTCCACGCCCTGCATCAACTGGGTGCAAGNACGACTGCGAACA GCGACCGTTTTACNTTCAGGCGCTTGCTGCGGGCCCGGCG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_012289 unedited GCACGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAT TAGTCTGTTCTTTATTTTCCTTGGAAAATTACAGGGTTACTTATCAACAAAATAACTGTC CCTCCGGGGGGGAAGTGCCATCCGGTGGCTTCTATGTACAGGCCCATCCCTGGGAACCAC ATTTCCAAAGAGGGGCCTCCCCCTGAGGCCCCCATTGGCCCCCTCCCAGGTATCCAAGAA TAAATCACATGGTGACAGCTGCCGGCATGGGTGAGTGGGACCCAAGCCTATTACCCGGCC AGAGGGTTTGGGGGCCTCTCTCCTGGAAGCCTGCTCTTTCCACACCCCCTTTCCCAGCCA GGCTGTCTTGGACACTCCCGGGGCTCCGCTGAGGGGCACATGATTCCCGCTTTGGACTTC TTTTGAGATGTCATTTTAACACTGAGGCATCCTGGCCTCCCTTCCCGGAAAATGGGTTAT TTGCAGAGCTGGCTTTTCTTTTAATCCCGGTTTTTGTACAAAAACAATGATACTCCCCAT TGGACTGTATTTTTGCCCAAAAAACAAAAGTGCCTCAACAGGTACAGTTCTGCTGGGCAA TCTGCTTCCCGGAGGGCTCCATGGTGACAGCCACCCCCACCCCACTCCCGCCCCGATGGC TTTCCGGTCACCTCGCCCCAAGTGGCTTGATTCTGGGCCGAACCCTCCACACTGGTCCGG AACCTGTGACCCTCTTAGCCCCAAGGCCTAAATTCTCCCCTGGGGCCAAGGATCCCCAGG CCCTTTCCGGGCCTTTGGGGCCTCCAAACTCCCGTCTCTTTTCACCACAAACGCCCCCCC TGTCACCGGGCCCGCCCCCATCCCCCCCCCCTAATACCCCCGGACAGACCAAACCCCTCC CCTTCCGGAGGGCCCCTGCTCTACCCCCGCACCCCCCCTTTGGGGTAGAACCCCACGAAT ACGCCGTCGCCG
Restriction Sites	NotI-NotI
ACCN	NM_012289
Insert Size	2600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012289.3, NP_036421.2
RefSeq Size	2577 bp
RefSeq ORF	1875 bp
Locus ID	9817
UniProt ID	Q14145
Domains	BTB, Kelch
Protein Families	Transcription Factors
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) has an alternate 5' UTR, as compared to variant 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (3)

The use of this cDNA Clones has been cited in the following citations:
Rotavirus NSP1 associates with components of the cullin RING ligase family of E3 ubiquitin ligases ,Lutz, LM;Pace, CR;Arnold, MM;, J. Virol. ,PubMed ID 27099313 [KEAP1]
KEAP1 Is a Redox Sensitive Target That Arbitrates the Opposing Radiosensitive Effects of Parthenolide in Normal and Cancer Cells ,Yong Xu, Fang Fang, Sumitra Miriyala, Peter A. Crooks, Terry D. Oberley, Luksana Chaiswing, Teresa Noel, Aaron K. Holley, Yanming Zhao, Kelley K. Kiningham, Daret K. St. Clair, and William H. St. Clair, Cancer Res., Jul 2013; 73: 4406 - 4417. ,PubMed ID 23674500 [KEAP1]
Dysfunctional KEAP1–NRF2 Interaction in Non-Small-Cell Lung Cancer ,Singh A, Misra V, Thimmulappa RK, Lee H, Ames S, et al., PLoS Med 3(10): e420. doi:10.1371/journal.pmed.0030420 [KEAP1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC202513	KEAP1 (Myc-DDK-tagged)-Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,990.00
RC202513L1	Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC202513L2	Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC202513L3	Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC202513L4	Lenti ORF clone of Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG202513	KEAP1 (tGFP-tagged) - Human kelch-like ECH-associated protein 1 (KEAP1), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,370.00

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