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DHCR7 (NM_001360) Human Untagged Clone
CAT＃: SC110871
DHCR7 (untagged)-Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SLOS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110871 sequence for NM_001360 edited (data generated by NextGen Sequencing)
ATGGCTGCAAAATCGCAACCCAACATTCCCAAAGCCAAGAGTCTAGATGGCGTCACCAAT GACAGAACCGCATCTCAAGGGCAGTGGGGCCGTGCCTGGGAGGTGGACTGGTTTTCACTG GCGAGCGTCATCTTCCTACTGCTGTTCGCCCCCTTCATCGTCTACTACTTCATCATGGCT TGTGACCAGTACAGCTGCGCCCTGACTGGCCCTGTGGTGGACATCGTCACCGGACATGCT CGGCTCTCGGACATCTGGGCCAAGACTCCACCTATAACGAGGAAAGCCGCCCAGCTCTAT ACCTTGTGGGTCACCTTCCAGGTGCTTCTGTACACGTCTCTCCCTGACTTCTGCCATAAG TTTCTACCCGGCTACGTAGGAGGCATCCAGGAGGGGGCCGTGACTCCTGCAGGGGTTGTG AACAAGTATCAGATCAATGGCCTGCAAGCCTGGCTCCTCACGCACCTGCTCTGGTTTGCA AACGCTCATCTCCTGTCCTGGTTCTCGCCCACCATCATCTTCGACAACTGGATCCCACTG CTGTGGTGCGCCAACATCCTTGGCTATGCCGTCTCCACCTTCGCCATGGTCAAGGGCTAC TTCTTCCCCACCAGCGCCAGAGACTGCAAATTCACAGGCAATTTCTTTTACAACTACATG ATGGGCATCGAGTTTAACCCTCGGATCGGGAAGTGGTTTGACTTCAAGCTGTTCTTCAAT GGGCGCCCCGGGATCGTCGCCTGGACCCTCATCAACCTGTCCTTCGCAGCGAAGCAGCGG GAGCTCCACAGCCATGTGACCAATGCCATGGTCCTGGTCAACGTCCTGCAGGCCATCTAC GTGATTGACTTCTTCTGGAACGAAACCTGGTACCTGAAGACCATTGACATCTGCCATGAC CACTTCGGGTGGTACCTGGGCTGGGGCGACTGTGTCTGGCTGCCTTATCTTTACACGCTG CAGGGTCTGTACTTGGTGTACCACCCCGTGCAGCTGTCCACCCCGCACGCCGTGGGCGTC CTGCTGCTGGGCCTGGTGGGCTACTACATCTTCCGGGTGGCCAACCACCAGAAGGACCTG TTCCGCCGCACGGATGGGCGCTGCCTCATCTGGGGCAGGAAGCCCAAGGTCATCGAGTGC TCCTACACATCCGCCGATGGGCAGAGGCACCACAGCAAGCTGCTGGTGTCGGGCTTCTGG GGCGTGGCCCGCCACTTCAACTACGTCGGCGACCTGATGGGCAGCCTGGCCTACTGCCTG GCCTGTGGCGGCGGCCACCTGCTGCCCTACTTCTACATCATCTACATGGCCATCCTGCTG ACCCACCGCTGCCTCCGGGACGAGCACCGCTGCGCCAGCAAGTACGGCCGGGACTGGGAG CGCTACACCGCCGCAGTGCCTTACCGCCTGCTGCCTGGAATCTTCTAA Clone variation with respect to NM_001360.2 >OriGene 5' read for NM_001360 unedited
NGGGCAAAATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGCCTCGTGCC GAATTCGGCACGAGGGTGGAGCAGCGCGCGCAAGCGAGGCCAGGGGAAGGTGGGCGCAGG TGAGGGGCCGAGGTGTGCGCAGGACTTTAGCCGGTTGAGAAGGATCAAGCAGGCATTTGG AGCACAGGTGTCTAGAAACTTTTAAGGGGCCGGTTCAAGAAGGAAAAGTTCCCTTCTGCT GTGAAACTATTTGGCAAGAGGCTGGAGGGCCCAATGGCTGCAAAATCGCAACCCAACATT CCCAAAGCCAAGAGTCTAGATGGCGTCACCAATGACAGAACCGCATCTCAAGGGCAGTGG GGCCGTGCCTGGGAGGTGGACTGGTTTTCACTGGCGAGCGTCATCTTCCTACTGCTGTTC GCCCCCTTCATCGTCTACTACTTCATCATGGCTTGTGACCAGTACAGCTGCGCCCTGACT GGCCCTGTGGTGGACATCGTCACCGGACATGCTCGGCTCTCGGACATCTGGGCCAAGACT CCACCTATAACGAGGAAAGCCGCCCAGCTCTATACCTTGTGGGTCACCTTCCAGGTGCTT CTGTACACGTCTCTCCCTGACTTCTGCCATAAGTTTCTACCCGGCTACGTAGGAGGCATC CAGGAGGGGGCCGTGACTCCTGCAGGGGTTGTGAACAAGTATCAGATCAATGGCCTGCAA GCCTGGCTCCTCACGCACCTGCTCTGGTTTGCAAACGCTCATCTCCTGTCCTGGTTCTCG CCCACCATCATCTTCGACAACTGGATCCCACTGCTGTGTTGCGCCCACATCCTTGGCTAT GCCGTCTCCACCTTCGNCATGGTCAAGGGCTACTTTTTTCCCACCAGGGCCAGAGACTGG >OriGene 3' read for NM_001360 unedited
GGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTCTTTTTTTCTTTTTTCTT TTTTTTCTTTTTTTCTTTTTTTTTCTTGAACAGGGGGAAATTTAATTTAAAAATGAACTC TTCTCGGAGCCTAAAGTTTAAAAAAAGTTTTACCCAACCCCCTTGGGGTGAGGAAAAATG CCCCAGGGGAAAAAAGCCCAAAAAAGGGGGGGCCCTCCCGAGGGTGGGACTGAAACCTCC TCCCTGGAAAAAGGGGGGGAGGCCCCTGAGGGGGAATTTCCCCGGGTTGGTTGAGCCAAA TTTTGCCCAGTCTTTGGGCAAGCAAAAAATTTTTTCGAAAGGGGGGGCGCTCCCAGGGAA TTGGGAAACAAAACCCCCCTGCCTTCCCCTGAAAGGGCCCAGCCGGGGCTGGGACCTTTT TTAGGGCTGCAAATTCCAGGCAAAACACTCCTGGCAGCTGTGCCCCAGGATCCCGAAGGA AACCACCTGAAAGCCCCTTTTTTCCCATGGTCCCAGTTTAACACCGGGCCCCCTCCCAAG GGAAAAACGGGCACGGGCCCCCCCCACCATTGTATAATTCGCACGCGAGGGGGTTTCTTA TCTCGTAGGCCCTTCCTGGATTCCCCCCCGCCCCCCTCTCCCTTTTTTTTACCACTGATT AATTGCCTCTATTGAAAGGGCGTGGGAAACCCCTCTGTTCCCCATTGGGCCTAACTGATC CTGTCTCCGTCTCCTAAACGGCAATTTTCTTCCCAGGACTTCTTTAGGTCCCTGTGTTAT CCTTTGCCCCCAGATTCCGTTTTTAAATTTACTCTTTGTCGGTACTCCCCATTTCCTCCA CCTCCGTCTCCTTATTGCATTACTTTCTAAAACTCCTTTTCATCTTCACTCTTCACTATG CCCACTTTCTCTCCTCATACCCCATCTTGTCTCCCCGCTGACCTTATTCTCCTTATCTTG TTTATCCGTGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001360 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001360.1, NP_001351.1 |
| RefSeq Size | 2597 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 1717 |
| UniProt ID | Q9UBM7 |
| Domains | ERG4_ERG24 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Metabolic pathways, Steroid biosynthesis |
| Gene Summary | This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Identification of a tumor-promoter cholesterol metabolite in human breast cancers acting through the glucocorticoid receptor
,Voisin, M;de Medina, P;Mallinger, A;Dalenc, F;Huc-Claustre, E;Leignadier, J;Serhan, N;Soules, R;Ségala, G;Mougel, A;Noguer, E;Mhamdi, L;Bacquié, E;Iuliano, L;Zerbinati, C;Lacroix-Triki, M;Chaltiel, L;Filleron, T;Cavaillès, V;Al Saati, T;Rochaix, P;Duprez-Paumier, R;Franchet, C;Ligat, L;Lopez, F;Record, M;Poirot, M;Silvente-Poirot, S;,
Proc. Natl. Acad. Sci. U.S.A.
,PubMed ID 29078321
[DHCR7]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200480 | DHCR7 (Myc-DDK-tagged)-Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1 |
CNY 3,656.00 |
|
| RC200480L1 | Lenti ORF clone of Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200480L2 | Lenti ORF clone of Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200480L3 | Lenti ORF clone of Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200480L4 | Lenti ORF clone of Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200480 | DHCR7 (tGFP-tagged) - Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1 |
CNY 4,370.00 |
