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BUB1 (NM_004336) Human Untagged Clone
CAT＃: SC110841
BUB1 (untagged)-Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1)
CNY 7,560.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BUB1A; BUB1L; hBUB1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_004336 edited
ATGGACACCCCGGAAAATGTCCTTCAGATGCTTGAAGCCCACATGCAGAGCTACAAGGGC AATGACCCTCTTGGTGAATGGGAAAGATACATACAGTGGGTAGAAGAGAATTTTCCTGAG AATAAAGAATACTTGATAACTTTACTAGAACATTTAATGAAGGAATTTTTAGATAAGAAG AAATACCACAATGACCCAAGATTCATCAGTTATTGTTTAAAATTTGCTGAGTACAACAGT GACCTCCATCAATTTTTTGAGTTTCTGTACAACCATGGGATTGGAACCCTGTCATCCCCT CTGTACATTGCCTGGGCGGGGCATCTGGAAGCCCAAGGAGAGCTGCAGCATGCCAGTGCT GTCCTTCAGAGAGGAATTCAAAACCAGGCTGAACCCAGAGAGTTCCTGCAACAACAATAC AGGTTATTTCAGACACGCCTCACTGAAACCCATTTGCCAGCTCAAGCTAGAACCTCAGAA CCTCTGCATAATGTTCAGGTTTTAAATCAAATGATAACATCAAAATCAAATCCAGGAAAT AACATGGCCTGCATTTCTAAGAATCAGGGTTCAGAGCTTTCTGGAGTGATATCTTCAGCT TGTGATAAAGAGTCAAATATGGAACGAAGAGTGATCACGATTTCTAAATCAGAATATTCT GTGCACTCATCTTTGGCATCCAAAGTTGATGTTGAGCAGGTTGTTATGTATTGCAAGGAG AAGCTTATTCGTGGGGAATCAGAATTTTCCTTTGAAGAATTGAGAGCCCAGAAATACAAT CAACGGAGAAAGCATGAGCAATGGGTAAATGAAGACAGACATTATATGAAAAGGAAAGAA GCAAATGCTTTTGAAGAACAGCTATTAAAACAGAAAATGGATGAACTTCATAAGAAGTTG CATCAGGTGGTGGAGACATCCCATGAGGATCTGCCCGCTTCCCAGGAAAGGTCCGAGGTT AATCCAGCACGTATGGGGCCAAGTGTAGGCTCCCAGCAGGAACTGAGAGCGCCATGTCTT CCAGTAACCTATCAGCAGACACCAGTGAACATGGAAAAGAACCCAAGAGAGGCACCTCCT GTTGTTCCTCCTTTGGCAAATGCTATTTCTGCAGCTTTGGTGTCCCCAGCCACCAGCCAG AGCATTGCTCCTCCTGTTCCTTTGAAAGCCCAGACAGTAACAGACTCCATGTTTGCAGTG GCCAGCAAAGATGCTGGATGTGTGAATAAGAGTACTCATGAATTCAAGCCACAGAGTGGA GCAGAGATCAAAGAAGGGTGTGAAACACATAAGGTTGCCAACACAAGTTCTTTTCACACA ACTCCAAACACATCACTGGGAATGGTTCAGGCAACGCCATCCAAAGTGCAGCCATCACCC ACCGTGCACACAAAAGAAGCATTAGGTTTCATCATGAATATGTTTCAGGCTCCTACACTT CCTGATATTTCTGATGACAAAGATGAATGGCAATCTCTAGATCAAAATGAAGATGCATTT GAAGCCCAGTTTCAAAAAAATGTAAGGTCATCTGGGGCTTGGGGAGTCAATAAGATCATC TCTTCTTTGTCATCTGCTTTTCATGTGTTTGAAGATGGAAACAAAGAAAATTATGGATTA CCACAGCCTAAAAATAAACCCACAGGAGCCAGGACCTTTGGAGAACGCTCTGTCAGCAGA CTTCCTTCAAAACCAAAGGAGGAAGTGCCTCATGCTGAAGAGTTTTTGGATGACTCAACT GTATGGGGTATTCGCTGCAACAAAACCCTGGCACCCAGTCCTAAGAGCCCAGGAGACTTC ACATCTGCTGCACAACTTGCGTCTACACCATTCCACAAGCTTCCAGTGGAGTCAGTGCAC ATTTTAGAAGATAAAGAAAATGTGGTAGCAAAACAGTGTACCCAGGCGACTTTGGATTCT TGTGAGGAAAACATGGTGGTGCCTTCAAGGGATGGAAAATTCAGTCCAATTCAAGAGAAA AGCCCAAAACAGGCCTTGTCGTCTCACATGTATTCAGCATCCTTACTTCGTCTGAGCCAG CCTGCTGCAGGTGGGGTACTTACCTGTGAGGCAGAGTTGGGCGTTGAGGCTTGCAGACTC ACAGACACTGACGCTGCCATTGCAGAAGATCCACCAGATGCTATTGCTGGGCTCCAAGCA GAATGGATGCAGATGAGTTCACTTGGGACTGTTGATGCTCCAAACTTCATTGTTGGGAAC CCATGGGATGATAAGCTGATTTTCAAACTTTTATCTGGGCTTTCTAAACCAGTGAGTTCC TATCCAAATACTTTTGAATGGCAATGTAAACTTCCAGCCATCAAGCCCAAGACTGAATTT CAATTGGGTTCTAAGCTGGTCTATGTCCATCACCTTCTTGGAGAAGGAGCCTTTGCCCAG GTGTACGAAGCTACCCAGGGAGATCTGAATGATGCTAAAAATAAACAGAAATTTGTTTTA AAGGTCCAAAAGCCTGCCAACCCCTGGGAATTCTACATTGGGACCCAGTTGATGGAAAGA CTAAAGCCATCTATGCAGCACATGTTTATGAAGTTCTATTCTGCCCACTTATTCCAGAAT GGCAGTGTATTAGTAGGAGAGCTCTACAGCTATGGAACATTATTAAATGCCATTAACCTC TATAAAAATACCCCTGAAAAAGTGATGCCTCAAGGTCTTGTCATCTCTTTTGCTATGAGA ATGCTTTACATGATTGAGCAAGTGCATGACTGTGAAATCATTCATGGAGACATTAAACCA GACAATTTCATACTTGGAAACGGATTTTTGGAACAGGATGATGAAGATGATTTATCTGCT GGCTTGGCACTGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTTCCAAAAGGAACT ATATTCACAGCAAAGTGTGAAACATCTGGTTTTCAGTGTGTTGAGATGCTCAGCAACAAA CCATGGAACTACCAGATCGATTACTTTGGGGTTGCTGCAACAGTATATTGCATGCTCTTT GGCACTTACATGAAAGTGAAAAATGAAGGAGGAGAGTGTAAGCCTGAAGGTCTTTTTAGA AGGCTTCCTCATTTGGATATGTGGAATGAATTTTTTCATGTTATGTTGAATATTCCAGAT TGTCATCATCTTCCATCTTTGGATTTGTTAAGGCAAAAGCTGAAGAAAGTATTTCAACAA CACTATACTAACAAGATTAGGGCCCTACGTAATAGGCTAATTGTACTGCTCTTAGAATGT AAGCGTTCACGAAAATAA >OriGene 5' read for NM_004336 unedited
GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTTCTAG TTTGCGGTTCAGGTTTGGCCGCTGCCGGCCAGCGTCCTCTGGCCATGGACACCCCGGAAA ATGTCCTTCAGATGCTTGAAGCCCACATGCAGAGCTACAAGGGCAATGACCCTCTTGGTG AATGGGAAAGATACATACAGTGGGTAGAAGAGAATTTTCCTGAGAATAAAGAATACTTGA TAACTTTACTAGAACATTTAATGAAGGAATTTTTAGATAAGAAGAAATACCACAATGACC CAAGATTCATCAGTTATTGTTTAAAATTTGCTGAGTACAACAGTGACCTCCATCAATTTT TTGAGTTTCTGTACAACCATGGGATTGGAACCCTGTCATCCCCTCTGTACATTGCCTGGG CGGGGCATCTGGAAGCCCAAGGAGAGCTGCAGCATGCCAGTGCTGTCCTTCAGAGAGGAA TTCAAAACCAGGCTGAACCCAGAGAGTTCCTGCAACAACAATACAGGTTATTTCAGACAC GCCTCACTGAAACCCATTTGCCAGCTCAAGCTAGAACCTCAGAACCTCTGCATAATGTTC AGGTTTTAAATCAAATGATAACATCAAAATCAAATCCAGGAAATAACATGGCCTGCATTT CTAAGAATCANGGTTCAGAGCTTTCTGGAGTGATATCTTCAGCTTGTGATAAAGAGTCAA ATATGGAACGAAGAGTGATCACGATTTCTAAATCAGAATATTCTGTGCACTCATCTTTGG CATCCAAAGTTGATGTTGAGCANGTTTGTATGTATTGCAAGGAGAGCTTATTCGTGGGGA TCAGAATTTNCTTTTGAGATTGNAGAGCCAGAATACAATCAACGAGAAAGCATGAGNCAT GGGNNTAATGAGACAGACATTNTTGAAAGGNAAAGAGCAATGCTTTTGAGACAGCTATTA AACAGAAATGTTGAACTCATAGAAGTGCTAN >OriGene 3' read for NM_004336 unedited
AATGGGTGTTGCCATGGGCATTTTTTNTTTTAACAAACATTTACATAAACAATAAATGAA AAAAAAACAGGTTTAAAGTGAGCAGATTCATATTTACAGTGTGATTTTTAAGGACTGTCT ATATCCAAATTTTATTTTCGTGAACGCTTACATTCTAAGAGCAGTACAATTAGCCTATTA CGTAGGGCCCTAATCTTGTTAGTATAGTGTTGTTGAAATACTTTCTTCAGCTTTTGCCTT AACAAATCCAAAGATGGAAGATGATGACAATCTGGAATATTCAACATAACATGAAAAAAT TCATTCCACATATCCAAATGAGGAAGCCTTCTAAAAAGACCTTCAGGCTTACACTCTCCT CCTTCATTTTTCACTTTCATGTAAGTGCCAAAGAGCATGCAATATACTGTTGCAGCAACC CCAAAGTAATCGATCTGGTAGTTCCATGGTTTGTTGCTGAGCATCTCAACACACTGAAAA CCAGATGTTTCACACTTTGCTGTGAATATAGTTCCTTTTGGAAAAAGTTTCATATCTATA CTCTGACCCAGGTCAATCAGGTGCCAAGCCAGCGGATAAATCATCTTCATCATCCTGTTC CAAAAATCCGTTTCCAAGTATGAAATTGTCTGGTTTAATGTCCTCCTGGAATGATTTTCC AGTCATGCACTTGCTCAATCATGTTAAGCATTCTCATAGCAAAAGAGATGAACAGACCTT GAGGCATCACTTTTTCAGGGGTATTTTTTATAGAGGTTAATGGCCATTTATAATGGTTCC AAGCTGGTGGAGCTTCCTACTAATACACTGGCCTTTTGGAATAAGGGGGCAGAAAAGGAA CTTTCAAACATGTGCGGCATAAAAGGCTTTAGGCTTCCAATAACGGGGCCCAAGTGAAAT ATCCGGGGGTTGGCAGACTTTGGACCTTAAACAAATTTTGTTTTTTTTAGCTACTTCCAT CTCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004336 |
| Insert Size | 3900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004336.2, NP_004327.1 |
| RefSeq Size | 3486 bp |
| RefSeq ORF | 3258 bp |
| Locus ID | 699 |
| UniProt ID | O43683 |
| Domains | pkinase, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation |
| Gene Summary | This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207013 | BUB1 (Myc-DDK-tagged)-Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) |
CNY 7,552.00 |
|
| RC207013L1 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), Myc-DDK-tagged |
CNY 9,952.00 |
|
| RC207013L2 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), mGFP tagged |
CNY 9,952.00 |
|
| RC207013L3 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC207013L4 | Lenti ORF clone of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), mGFP tagged |
CNY 10,260.00 |
|
| RG207013 | BUB1 (tGFP-tagged) - Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) |
CNY 9,152.00 |
|
| SC323526 | BUB1 (untagged)-Kinase deficient mutant (K821M) of Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) |
CNY 7,560.00 |
