PDE4DIP (BC005949) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC005949, the custom clone sequence may differ by one or more nucleotides AGAGCAGTGAGCCCAGTCCCGAGTCCCGGAGAGAGCTCCAGCAATAGGGGCCATGTCGCCATAGCCCCAG CCTCTCGGTCCGCAGCCTCAGCAGCGTCCCAGCCGGCTGGCTTCATGCTGCGGTGCAGCTGCACCATGTT CCTGGGTTGAGGGGGCAATCGGGCACGCTCCTCCCCATGGGTTGCCCATCATGTCTAATGGATATCGCAC TCTGTCCCAGCACCTCAATGACCTGAAGAAGGAGAACTTCAGCCTCAAGCTGCTCATCTACTTCCTGGAG GAGCGCATGCAACAGAAGTATGAGGCCAGCCGGGAGGACATCTACAAGCGGAACACTGAGCTGAAGGTTG AAGTGGAGAGCTTGAAACGAGAACTCCAGGACAAGAAACAGCATCTGGATAAAACATGGGCTGATGTGGA GAATCTCAACAGTCAGAATGAAGCTGAGCTCCGACGCCAGTTTGAGGAGCGACAGCAGGAGACGGAGCAT GTTTATGAGCTCTTGGAGAATAAGATCCAGCTTCTGCAGGAGGAATCCAGGCTAGCAAAGAATGAAGCTG CGCGGATGGCAGCTCTGGTGGAAGCAGAGAAGGAGTGTAACCTGGAGCTCTCAGAGAAACTGAAGGGAGT CACCAAAAACTGGGAAGATGTACCAGGAGACCAGGTCAAGCCCGACCAATACACTGAGGCCCTGGCCCAG AGGGACAATCCTTCAACTGACAGCTTAAAGAACTTCAGGTTGTTCTGATTCTTGAGCCTCCTCTACAGCT TCAGAGAGGACTTTCATTTTATTTTGGATCAAATGCTCCACAACTAGTTGAAACTGGAATTAAATTTTAT ATGAAGTTCCTAGATGATTTAAAGCTGTAAGAAGAAGAATAATGAATCATAAGAAAACTTGCTGCTACAG ACATCAAAAAGGAATGTTACCATCCCTCATGCTAATCCTTTTCATTTTAAATAAACAGGATCTAAAAAAA ATAATGCTGGGAAGTCCTAACCACATCAAGAATGCCTCAGATCAGTGACCCAAGGAACCTTCCAGAATGG ATGAAATAGACCCAAAGCCGAATTCACCTAATTTTAGGGCCAAAAACCCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC005949 unedited TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTCACGCAGCATGT TCCTCTGTCAGGGATTCCGCAAATATCTCCCTGAGGTAAAAAAGGAAAGTGTGCTGCGCT CCAGCACCCAGAGCAGTGAGCCCAGTCCCGAGTCCCGGAGAGAGCTCCAGCAATAGGGGC CATGTCGCCATAGCCCCAGCCTCTCGGTCCGCAGCCTCAGCAGCGTCCCAGCCGGCTGGC TTCATGCTGCGGTGCAGCTGCACCATGTTCCTGGGTTGAGGGGGCAATCGGGCACGCTCC TCCCCATGGGTTGCCCATCATGTCTAATGGATATCGCACTCTGTCCCAGCACCTCAATGA CCTGAAGAAGGAGAACTTCAGCCTCAAGCTGCGCATCTACTTCCTGGAGGAGCGCATGCA ACAGAAGTATGAGGCCAGCCGGGAGGACATCTACAAGCGGAACACTGAGCTGAAGGTTGA AGTGGAGAGCTTGAAACGAGAACTCCAGGACAAGAAACAGCATCTGGATAAAACATGGGC TGATGTGGAGAATCTCAACAGTCAGAATGAAGCTGAGCTCCGACGCCAGTTTGAGGAGCG ACAGCAGGAGACGGAGCATGTTTATGAGCTCTTGGAGAATAAGATGCAGCTTCTGCAGGA GGAATCCNAGCTAGCANAGAATGAAGCTGCGCGGATGGCAGCTCTGGTGGAAGCAGAGAA GGAGTGTAACCTGGAGCTCTCAGAGAAACTGAAGGGAGTCACCANNAACTGGGAAGATGT ACCNAGAGACCANNGTCAGCCCGACCAATACACTNGAGGCCCTGCCCCAGAGGGNACAGT AGGTGCCTTNCGGNGCTCTTTNTGCCGCTTGTCTTTNGCCATTCTCAGGCATACACAGCT GTCCTGGTCCCTTTCAGGACTGACAGTAGA
Restriction Sites	NotI-NotI
ACCN	BC005949
Insert Size	4100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC005949.1
RefSeq Size	1130 bp
RefSeq ORF	1130 bp
Locus ID	9659
Gene Summary	The protein encoded by this gene serves to anchor phosphodiesterase 4D to the Golgi/centrosome region of the cell. Defects in this gene may be a cause of myeloproliferative disorder (MBD) associated with eosinophilia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]

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