KCNMA1 (BC024965) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA205K10.1; BKTM; hSlo; KCa1.1; MaxiK; mSLO1; SAKCA; SLO; SLO-ALPHA; SLO1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC024965, the custom clone sequence may differ by one or more nucleotides CGCCAGGTCGCGCACAGCGCCCCGAGCCCAGGCGCCTCCCCGCCCCCCTCCCGCGCTCCGCGGCGGCGGC GGCGGCGGCAGCAGTAGCAGCAATATGGCTGTTGATGGGTGTTTGGGGTGGCGCTGGCGGCGGGAGGAGC TCCCCCGAGCCCCTGCGCCGGCTGCCCGTTGCTAGCTATGGCAAATGGTGGCGGCGGCGGCGGCGGCAGC AGCGGCGGCGGCGGCGGCGGCGGAGGCAGCAGTCTTAGAATGAGTAGCAATATCCACGCGAACCATCTCA GCCTAGACGCGTCCTCCTCCTCCTCCTCCTCCTCTTCCTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTC GTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATCATCCCGGTGACCATGGAGGTGCCGTGCGACAGC CGGGGCCAACGCATGTGGTGGGCTTTCCTGGCCTCCTCCATGGTGACTTTCTTCGGGGGCCTCTTCATCA TCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGTGTGCTGCCACTGCGGGGGCAAGACGAAGGCCAC CCACTTTGGGTCCCCGGAAATGCCACCAGCAGCGCGGAGCTGGAGCGGGAGTCCGCCTGAGGCCGCGGTT TTACGCGGAGCGTCTTCCCTGGCGCTCGAGGTGGCTAGATGTCGTCGGCTTTAGCTATTCCTGGTGCAGA CGCCCACCGGCACTGAGGCTGGCGACACCTTCGATGTATGGTTGCTAGGCTCACTGCCTGGTCTGTAGTC GTGTCCCTAGAGATGTCTTCCCTTCCCCACAGCCACTGGCTGGAGTGGGGGACCCGACACTGATAGAAAA CTGCAGAATAAAAAAGAAAGAACTGGAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC024965 unedited GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCCCGCCCCCCT CCCGCGCTCCGCGGCGGCGGCGGCGGCGGCAGCAGTAGCAGCAATATGGCTGTTGATGGG TGTTTGGGGTGGCGCTGGCGGCGGGAGGAGCTCCCCCGAGCCCCTGCGCCGGCTGCCCGT TGCTAGCTATGGCAAATGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCG GCGGAGGCAGCAGTCTTAGAATGAGTAGCAATATCCACGCGAACCATCTCAGCCTAGACG CGTCCTCCTCCTCCTCCTCCTCCTCTTCCTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCT CGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATCATCCCGGTGACCATGGAGGTGC CGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCCTGGCCTCCTCCATGGTGACTT TCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGTGT GCTGCCACTGCGGGGGCAAGACGAAGGTAACGCGCGCCCCGGGCGCCCGCCCTCTGCGCC AGCCCCACCGCTTCTCGGCGTCCCCTGCAGCCCACCTCCTCGCTCCGCGCCGCGCCGTGC CTCCCTCCATCCCCGCCTGCCTTCCCCTCGCCGCCTTCCCTTCCCTCGGCTCTACCGGTG CTCTCGTATTCGGGCAACCGGCGGCGCGCCCCGGGGTGAGGAGTTGCCCCCAAGCAGTGC GCTCCCGGACGCGGACGCTCAAAACTGTCCTCTTGTTTATGTAAGGTTTGTGGCCGCTGC GGCTGGGGCCCGGTCCGGGACTCCCAGCTCGGTTGCCCGGTCCGGGTGGGGACACCCATC ACCGGGACGGAAGAACTGGGGCACTGGTGCCTGTTTGACCGTTCCCTGCGGGCTTGGCTC TGGGGGGGGAAAAGGAACACN
Restriction Sites	NotI-NotI
ACCN	BC024965
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC024965.1
RefSeq Size	882 bp
RefSeq ORF	882 bp
Locus ID	3778
Protein Families	Druggable Genome, Ion Channels: Potassium, Transmembrane
Protein Pathways	Vascular smooth muscle contraction
Gene Summary	MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

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