PIGX (AK000529) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PIG-X
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK000529, the custom clone sequence may differ by one or more nucleotides AGCACTCGGTCCCAGCCGATAAATCTGGGGCAGCGCGCGGTAGGAGCTGCGGGCGGCCAGGCCCCTTCCT GCGTCCGCACCTGGCCCCGCGCGCCCCTCTCGGGCGTCCGGCTTCCGGCGTCCTGGCGGCTCGGGTGGCG GCGGTTCGGGCGGCCGCCTGGCTGCTCCTCGGGGCGGCGACCGGGCTCACGCGCGGGCCCGCCGCGGCCT TCACCGCCGCGCGCTCTGACGCCGGCATAAGGGCCATGTGTTCTGAAATTATTTTGAGGCAAGAAGTTTT GAAAGATGGTTTCCACAGAGACCTTTTAATCAAAGTGAAGTTTGGGGAAAGCATTGAGGACTTGCACACC TGCCGTCTCTTAATTAAACAGGACATTCCTGCAGGACTTTATGTGGATCCGTATGAGTTGGCTTCATTAC GAGAGAGAAACATAACAGAGGCAGTGATGGTTTCAGAAAATTTTGATATAGAGGCCCCTAACTATTTGTC CAAGGAGTCTGAAGTTCTCATTTATGCCAGACGAGATTCACAGTGCATTGACTGTTTTCAAGCCTTTTTG CCTGTGCACTGCCGCTATCATCGGCCGCACAGTGAAGATGGAGAAGCCTCGATTGTGGTCAATAACCCAG ATTTGTTGATGTTTTGTGACCAAGCTGGCTCCAGAAGAATGATCAGATTCCGGTTTGATTCCTTTGACAA AACTATAGAGTTCCCGATTTTGAAATGCTGGGCTCACTCAGAAGTGGCAGCCCCTTGTGCTTTGGAGAAT GAGGATATCTGCCAATGGAACAAGATGAAGTATAAATCAGTATATAAGAATGTGATTCTACAAGTTCCAG TGGGACTGACTGTACATACCTCTCTAGTATGTTCTGTGACTCTGCTCATTACAATCCTGTGCTCTAAAAA AAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK000529 unedited ATACTTTACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC GCGAATTCGGCACGAGGCTCGGTCCCAGCCGATAAATCTGGGGCAGCGCGCGGTAGGAGC TGCGGGCGGCCAGGCCCCTTCCTGCGTCCGCACCTGGCCCCGCGCGCCCCTCTCGGGCGT CCGGCTTCCGGCGTCCTGGCGGCTCGGGTGGCGGCGGTTCGGGCGGCCGCCTGGCTGCTC CTCGGGGCGGCGACCGGGCTCACGCGCGGGCCCGCCGCGGCCTTCACCGCCGCGCGCTCT GACGCCGGCATAAGGGCCATGTGTTCTGAAATTATTTTGAGGCAAGAAGTTTTGAAAGAT GGTTTCCACAGAGACCTTTTAATCAAAGTGAAGTTTGGGGAAAGCATTGAGGACTTGCAC ACCTGCCGTCTCTTAATTAAACAGGACATTCCTGCAGGACTTTATGTGGATCCGTATGAG TTGGCTTCATTACGAGAGAGAAACATAACAGAGGCAGTGATGGTTTCAGAAAATTTTGAT ATAGAGGCCCCTAACTATTTGTCCAAGGAGTCTGAAGTTCTCATTTATGCCAGACGAGAT TCACAGTGCATTGACTGTTTTCAAGCCTTTTTGCCTGTGCACTGCCGCTATCATCGGCCG CACAGTGAAGATGGAGAAGCCTCGATTGTGGTCAATAACCCAGATTTGTTGATGTTTTGT GACCAAGCTGGCTCCAGAAGAATGATCAGATTCCGGTTTGATTCCTTTGACAAACTATAG AGTTCCCGATTTTGAATGCTGGGCTCACTCAGAAGTGGCAGCCCCTTGTGCTTTGGAGAA TGAGATATCTGCCATGGAACAAGA
Restriction Sites	NotI-NotI
ACCN	AK000529
Insert Size	2650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK000529.1, BAA91233.1
RefSeq Size	921 bp
RefSeq ORF	921 bp
Locus ID	54965
Protein Families	Transmembrane
Protein Pathways	Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
Gene Summary	This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

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